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Two closely related members of Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, reveal distinct functions in response to plant-parasitic nematode infection
Year:
2014
Source of publication :
Molecular Plant Pathology
Authors :
Horowitz, Sigal Brown
;
.
Iberkleid, Ionit
;
.
Ozalvo, Rachel
;
.
Volume :
15
Co-Authors:
Ozalvo, R., Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, 50250, Israel
Cabrera, J., Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, Toledo, 45071, Spain
Escobar, C., Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, Toledo, 45071, Spain
Christensen, S.A., Department of Plant Pathology and Microbiology, Texas A andM University, TAMU 2132, College Station, TX, 77843-2132, United States
Borrego, E.J., Department of Plant Pathology and Microbiology, Texas A andM University, TAMU 2132, College Station, TX, 77843-2132, United States
Kolomiets, M.V., Department of Plant Pathology and Microbiology, Texas A andM University, TAMU 2132, College Station, TX, 77843-2132, United States
Castresana, C., Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma, Cantoblanco, Madrid, E-28049, Spain
Iberkleid, I., Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, 50250, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Horowitz, S.B., Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
319
To page:
332
(
Total pages:
14
)
Abstract:
The responses of two closely related members of Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, to infection by the sedentary nematodes root-knot nematode (Meloidogyne javanica) and cyst nematode (Heterodera schachtii) were analysed in transgenic Arabidopsis seedlings. The tissue localization of LOX3 and LOX4 gene expression using β-glucuronidase (GUS) reporter gene constructs showed local induction of LOX3 expression when second-stage juveniles reached the vascular bundle and during the early stages of plant-nematode interaction through gall and syncytia formation. Thin sections of nematode-infested knots indicated LOX3 expression in mature giant cells, and high expression in neighbouring cells and those surrounding the female body. LOX4 promoter was also activated by nematode infection, although the GUS signal weakened as infection and disease progressed. Homozygous insertion mutants lacking LOX3 were less susceptible than wild-type plants to root-knot nematode infection, as reflected by a decrease in female counts. Conversely, deficiency in LOX4 function led to a marked increase in females and egg mass number and in the female to male ratio of M.javanica and H.schachtii, respectively. The susceptibility of lox4 mutants was accompanied by increased expression of allene oxide synthase, allene oxide cyclase and ethylene-responsive transcription factor 4, and the accumulation of jasmonic acid, measured in the roots of lox4 mutants. This response was not found in lox3 mutants. Taken together, our results reveal that LOX4 and LOX3 interfere differentially with distinct metabolic and signalling pathways, and that LOX4 plays a major role in controlling plant defence against nematode infection. © 2013 BSPP AND JOHN WILEY & SONS LTD.
Note:
Related Files :
Animal
Animals
arabidopsis
Female
Male
metabolism
Nematoda
Parasitology
Show More
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More details
DOI :
10.1111/mpp.12094
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21855
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
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Scientific Publication
Two closely related members of Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, reveal distinct functions in response to plant-parasitic nematode infection
15
Ozalvo, R., Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, 50250, Israel
Cabrera, J., Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, Toledo, 45071, Spain
Escobar, C., Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, Avenida de Carlos III s/n, Toledo, 45071, Spain
Christensen, S.A., Department of Plant Pathology and Microbiology, Texas A andM University, TAMU 2132, College Station, TX, 77843-2132, United States
Borrego, E.J., Department of Plant Pathology and Microbiology, Texas A andM University, TAMU 2132, College Station, TX, 77843-2132, United States
Kolomiets, M.V., Department of Plant Pathology and Microbiology, Texas A andM University, TAMU 2132, College Station, TX, 77843-2132, United States
Castresana, C., Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma, Cantoblanco, Madrid, E-28049, Spain
Iberkleid, I., Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, 50250, Israel, Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
Horowitz, S.B., Department of Entomology, Nematology and Chemistry Units, Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, 50250, Israel
Two closely related members of Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, reveal distinct functions in response to plant-parasitic nematode infection
The responses of two closely related members of Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, to infection by the sedentary nematodes root-knot nematode (Meloidogyne javanica) and cyst nematode (Heterodera schachtii) were analysed in transgenic Arabidopsis seedlings. The tissue localization of LOX3 and LOX4 gene expression using β-glucuronidase (GUS) reporter gene constructs showed local induction of LOX3 expression when second-stage juveniles reached the vascular bundle and during the early stages of plant-nematode interaction through gall and syncytia formation. Thin sections of nematode-infested knots indicated LOX3 expression in mature giant cells, and high expression in neighbouring cells and those surrounding the female body. LOX4 promoter was also activated by nematode infection, although the GUS signal weakened as infection and disease progressed. Homozygous insertion mutants lacking LOX3 were less susceptible than wild-type plants to root-knot nematode infection, as reflected by a decrease in female counts. Conversely, deficiency in LOX4 function led to a marked increase in females and egg mass number and in the female to male ratio of M.javanica and H.schachtii, respectively. The susceptibility of lox4 mutants was accompanied by increased expression of allene oxide synthase, allene oxide cyclase and ethylene-responsive transcription factor 4, and the accumulation of jasmonic acid, measured in the roots of lox4 mutants. This response was not found in lox3 mutants. Taken together, our results reveal that LOX4 and LOX3 interfere differentially with distinct metabolic and signalling pathways, and that LOX4 plays a major role in controlling plant defence against nematode infection. © 2013 BSPP AND JOHN WILEY & SONS LTD.
Scientific Publication
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