נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Purity control of F1‐hybrid tomato cultivars by RAPD markers
Year:
1995
Authors :
Gidoni, David
;
.
Pilowsky, Meir
;
.
Rom, M.
;
.
Volume :
114
Co-Authors:
Rom, M., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Bar, M., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Rom, A., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Pilowsky, M., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Gidoni, D., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
188
To page:
190
(
Total pages:
3
)
Abstract:
Randomly amplified polymorphic DNA (RAPD) markers were applied in purity control of hybrid seed production of tomato (Lycopersicon esculentum Mill.). DNA from three commercial F1‐hybrid cultivars and their parental lines was subjected to RAPD screening with 50 primers. Two of four primers which detected polymorphism between the parents tested, generated paternal‐specific RAPDs, enabling a clear distinction to be made between hybrids and their maternal parents. In addition, combination of the polymorphic DNA products generated by these primers exhibited hybrid‐specific patterns, enabling each cultivar to be identified. This result indicates the practical usefulness of RAPD markers in hybrid‐tomato‐seed purity‐control tests and cultivar identification. The approach is advantageous in its rapidity and simplicity, particularly as an alternative for those cultivars for which lengthy and costly phenotypic tests are currently used. Copyright © 1995, Wiley Blackwell. All rights reserved
Note:
Related Files :
hybrid purity
Lycopersicon esculentum
randomly amplified polymorphic DNA markers
Show More
Related Content
More details
DOI :
10.1111/j.1439-0523.1995.tb00790.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21859
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
Scientific Publication
Purity control of F1‐hybrid tomato cultivars by RAPD markers
114
Rom, M., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Bar, M., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Rom, A., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Pilowsky, M., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Gidoni, D., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization (ARO), Volcani Center, POB. 6, Bet Dagan, 50250, Israel
Purity control of F1‐hybrid tomato cultivars by RAPD markers
Randomly amplified polymorphic DNA (RAPD) markers were applied in purity control of hybrid seed production of tomato (Lycopersicon esculentum Mill.). DNA from three commercial F1‐hybrid cultivars and their parental lines was subjected to RAPD screening with 50 primers. Two of four primers which detected polymorphism between the parents tested, generated paternal‐specific RAPDs, enabling a clear distinction to be made between hybrids and their maternal parents. In addition, combination of the polymorphic DNA products generated by these primers exhibited hybrid‐specific patterns, enabling each cultivar to be identified. This result indicates the practical usefulness of RAPD markers in hybrid‐tomato‐seed purity‐control tests and cultivar identification. The approach is advantageous in its rapidity and simplicity, particularly as an alternative for those cultivars for which lengthy and costly phenotypic tests are currently used. Copyright © 1995, Wiley Blackwell. All rights reserved
Scientific Publication
You may also be interested in