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Tobacco mosaic virus (TMV) replicase and movement protein function synergistically in facilitating TMV spread by lateral diffusion in the plasmodesmal desmotubule of Nicotiana benthamiana
Year:
2008
Authors :
Guenoune (Gelbart), Dana
;
.
Volume :
21
Co-Authors:
Guenoune-Gelbart, D., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Elbaum, M., Department of Materials and Interfaces, Weizmann Institute, Rehovot, Israel
Sagi, G., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Levy, A., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Epel, B.L., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Facilitators :
From page:
335
To page:
345
(
Total pages:
11
)
Abstract:
Virus spread through plasmodesmata (Pd) is mediated by virus-encoded movement proteins (MPs) that modify Pd structure and function. The MP of Tobacco mosaic virus (TMVMP) is an endoplasmic reticulum (ER) integral membrane protein that binds viral RNA (vRNA), forming a vRNA:MP:ER complex. It has been hypothesized that TMVMP causes Pd to dilate, thus potentiating a cytoskeletal mediated sliding of the vRNA:MP:ER complex through Pd; in the absence of MP, by contrast, the ER cannot move through Pd. An alternate model proposes that cell-to-cell spread takes place by diffusion of the MP:vRNA complex in the ER membranes which traverse Pd. To test these models, we measured the effect of TMVMP and replicase expression on cell-to-cell spread of several green fluorescent protein-fused probes: a soluble cytoplasmic protein, two ER lumen proteins, and two ER membrane-bound proteins. Our data support the diffusion model in which a complex that includes ER-embedded MP, vRNA, and other components diffuses in the ER membrane within the Pd driven by the concentration gradient between an infected cell and adjacent noninfected cells. The data also suggest that the virus replicase and MP function together in altering Pd conductivity. © 2008 The American Phytopathological Society.
Note:
Related Files :
metabolism
Plasmodesmata
Tobacco mosaic virus
ultrastructure
Virology
virus RNA
Show More
Related Content
More details
DOI :
10.1094/MPMI-21-3-0335
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21899
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:47
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Scientific Publication
Tobacco mosaic virus (TMV) replicase and movement protein function synergistically in facilitating TMV spread by lateral diffusion in the plasmodesmal desmotubule of Nicotiana benthamiana
21
Guenoune-Gelbart, D., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Elbaum, M., Department of Materials and Interfaces, Weizmann Institute, Rehovot, Israel
Sagi, G., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Levy, A., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Epel, B.L., Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel
Tobacco mosaic virus (TMV) replicase and movement protein function synergistically in facilitating TMV spread by lateral diffusion in the plasmodesmal desmotubule of Nicotiana benthamiana
Virus spread through plasmodesmata (Pd) is mediated by virus-encoded movement proteins (MPs) that modify Pd structure and function. The MP of Tobacco mosaic virus (TMVMP) is an endoplasmic reticulum (ER) integral membrane protein that binds viral RNA (vRNA), forming a vRNA:MP:ER complex. It has been hypothesized that TMVMP causes Pd to dilate, thus potentiating a cytoskeletal mediated sliding of the vRNA:MP:ER complex through Pd; in the absence of MP, by contrast, the ER cannot move through Pd. An alternate model proposes that cell-to-cell spread takes place by diffusion of the MP:vRNA complex in the ER membranes which traverse Pd. To test these models, we measured the effect of TMVMP and replicase expression on cell-to-cell spread of several green fluorescent protein-fused probes: a soluble cytoplasmic protein, two ER lumen proteins, and two ER membrane-bound proteins. Our data support the diffusion model in which a complex that includes ER-embedded MP, vRNA, and other components diffuses in the ER membrane within the Pd driven by the concentration gradient between an infected cell and adjacent noninfected cells. The data also suggest that the virus replicase and MP function together in altering Pd conductivity. © 2008 The American Phytopathological Society.
Scientific Publication
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