אסיף מאגר המחקר החקלאי

Expression of a truncated tobacco NtCBP4 channel in transgenic plants and disruption of the homologous Arabidopsis CNGC1 gene confer Pb2+ tolerance

Year:

2000

Source of publication :

Authors :

Volume :

24

Co-Authors:

Sunkar, R., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Kaplan, B., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Bouché, N., INRA, Laboratoire de Biologie Cellulaire, Route de Saint Cyr, 78026 Versailles, France
Arazi, T., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Dolev, D., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Talke, I.N., Plant Laboratory, Department of Biology, University of York, York YO1 5YW, United Kingdom
Maathuis, F.J.M., Plant Laboratory, Department of Biology, University of York, York YO1 5YW, United Kingdom
Sanders, D., Plant Laboratory, Department of Biology, University of York, York YO1 5YW, United Kingdom
Bouchez, D., INRA, Laboratoire de Biologie Cellulaire, Route de Saint Cyr, 78026 Versailles, France
Fromm, H., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Centre for Plant Sciences, School of Biology, University of Leeds, Leeds LS2 9JT, United Kingdom, Centre for Plant Sciences, LIBA, United Kingdom

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Abstract:

Recently we reported on a plasma membrane tobacco protein (designated NtCBP4) that binds calmodulin. When overexpressed in transgenic plants, NtCBP4 confers Pb2+ hypersensitivity associated with enhanced accumulation of this toxic metal. To further investigate possible modulation of Pb2+ tolerance in plants, we prepared transgenic plants that express a truncated version of this protein (designated NtCBP4ΔC) from which its C-terminal, with the calmodulin-binding domain and part of the putative cyclic nucleotide-binding domain, was removed. In contrast to the phenotype of transgenic plants expressing the full-length gene, transgenic plants expressing the truncated gene showed improved tolerance to Pb2+, in addition to attenuated accumulation of this metal. Furthermore, disruption by T-DNA insertion mutagenesis of the Arabidopsis CNGC1 gene, which encodes a homologous protein, also conferred Pb2+ tolerance. We suggest that NtCBP4 and AtCNGC1 are components of a transport pathway responsible for Pb2+ entry into plant cells.

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