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Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation - (from Bio/Technology)
Year:
1993
Source of publication :
Bio/Technology
Authors :
Galili, Shmuel
;
.
Volume :
11
Co-Authors:
Perl, A., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel, Department of Fruit Tree Breeding and Genetics, The Volcani Center, Bet-Dagan, 50250, Israel
Galili, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Shaul, O., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Ben-Tzvi, I., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Galili, G., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Facilitators :
From page:
715
To page:
718
(
Total pages:
4
)
Abstract:
Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest. © 1993 Nature Publishing Group.
Note:
Related Files :
amino acid derivative
enzyme activity
gene expression
genetic transformation
Solanum tuberosum
threonine
Show More
Related Content
More details
DOI :
10.1038/nbt0693-715
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21979
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:48
Scientific Publication
Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation - (from Bio/Technology)
11
Perl, A., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel, Department of Fruit Tree Breeding and Genetics, The Volcani Center, Bet-Dagan, 50250, Israel
Galili, S., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Shaul, O., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Ben-Tzvi, I., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Galili, G., Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel
Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation
Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest. © 1993 Nature Publishing Group.
Scientific Publication
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