Itkin, M., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Heinig, U., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Tzfadia, O., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Bhide, A.J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Plant Molecular Biology Unit, Division of Biochemical Sciences, Council of Scientific and Industrial Research-National Chemical Laboratory, Pune 411008, MS, India
Shinde, B., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Plant Molecular Biology Unit, Division of Biochemical Sciences, Council of Scientific and Industrial Research-National Chemical Laboratory, Pune 411008, MS, India
Cardenas, P.D., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Bocobza, S.E., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Unger, T., Israel Structural Proteomics Center, Weizmann Institute of Science, Rehovot 76100, Israel
Malitsky, S., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Finkers, R., Plant Research International, Wageningen University and Research Centre, POB 16, 6700 AA, Wageningen, Netherlands
Tikunov, Y., Plant Research International, Wageningen University and Research Centre, POB 16, 6700 AA, Wageningen, Netherlands
Bovy, A., Plant Research International, Wageningen University and Research Centre, POB 16, 6700 AA, Wageningen, Netherlands
Chikate, Y., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Plant Molecular Biology Unit, Division of Biochemical Sciences, Council of Scientific and Industrial Research-National Chemical Laboratory, Pune 411008, MS, India
Singh, P., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Plant Molecular Biology Unit, Division of Biochemical Sciences, Council of Scientific and Industrial Research-National Chemical Laboratory, Pune 411008, MS, India
Rogachev, I., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel
Beekwilder, J., Plant Research International, Wageningen University and Research Centre, POB 16, 6700 AA, Wageningen, Netherlands
Giri, A.P., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel, Plant Molecular Biology Unit, Division of Biochemical Sciences, Council of Scientific and Industrial Research-National Chemical Laboratory, Pune 411008, MS, India
Aharoni, A., Department of Plant Sciences, Weizmann Institute of Science, Rehovot 76100, Israel

Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.