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Tissue culture of the alternate hosts of wheat, rye and oat rusts and their response to in vitro inoculation with the rust pathogens
Year:
1999
Authors :
Faingersh, Evgenia
;
.
Rotbaum, Arie
;
.
Zilkah, Shmuel
;
.
Volume :
59
Co-Authors:
Zilkah, S., Institute of Horticulture, Volcani Center, ARO, PO Box 6, Bet Dagan 50250, Israel
Faingersh, E., Institute of Horticulture, Volcani Center, ARO, PO Box 6, Bet Dagan 50250, Israel
Rotbaum, A., Institute of Horticulture, Volcani Center, ARO, PO Box 6, Bet Dagan 50250, Israel
Dvorkin, R., Inst. for Cereal Crop Improvement, Tel Aviv University, Ramat Aviv 69978, Israel
Eilam, T., Inst. for Cereal Crop Improvement, Tel Aviv University, Ramat Aviv 69978, Israel
Anikster, Y., Inst. for Cereal Crop Improvement, Tel Aviv University, Ramat Aviv 69978, Israel
Facilitators :
From page:
209
To page:
215
(
Total pages:
7
)
Abstract:
Alternate host plants of cereal rust fungi are necessary for studying the rust sexual cycle and pathogenicity. These plants are usually difficult to propagate through cloning, while seed-propagated plants may have variable responses to the pathogen. To overcome these obstacles, tissue culture, under controlled and aseptic conditions, was utilized for clonal propagation and in vitro inoculation of the following species: Rhamnus palaestinus Boiss., the alternate host of oat (Avena spp.) crown rust (Puccinia coronata Corda); Thalictrum speciosissimum L., the alternate host of brown leaf rust of wheat (Puccinia recondita f. sp. tritici Eriks. and Henn.); and Lycopsis arvensis L., the alternate host of rye (Secala spp.) leaf rust (Puccinia recondita f. sp. recondita Rob. and Desm.). Shoot culture procedures for initial establishment and proliferation were developed for all three alternate host species. Shoot cultures were multiplied at rates ranging from 0.3 to 1.7 shoots/week. Successful infection following inoculation with teliospores of the corresponding rust fungi was obtained for R. palaestinus and T. speciosissimum but not for L. arvensis. The hardening and acclimatization efficiency of rooted T. speciosissimum and L. arvensis was of 80-90%. The propagation efficiency for R. palaestinus was not successful because of the low rate and poor quality of its rooting. It is concluded that the in vitro system might be used as an alternative method for inoculation and multiplication of alternate hosts of cereal rusts, although more experimentation is needed to define accurately the appropriate conditions for the proper infection response.
Note:
Related Files :
Avena
cereal rust
fungal spore
Inoculation
leaf rust
Puccinia recondita
rooting
Secala spp.
wheat
Show More
Related Content
More details
DOI :
10.1023/A:1006402300429
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
22050
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:49
Scientific Publication
Tissue culture of the alternate hosts of wheat, rye and oat rusts and their response to in vitro inoculation with the rust pathogens
59
Zilkah, S., Institute of Horticulture, Volcani Center, ARO, PO Box 6, Bet Dagan 50250, Israel
Faingersh, E., Institute of Horticulture, Volcani Center, ARO, PO Box 6, Bet Dagan 50250, Israel
Rotbaum, A., Institute of Horticulture, Volcani Center, ARO, PO Box 6, Bet Dagan 50250, Israel
Dvorkin, R., Inst. for Cereal Crop Improvement, Tel Aviv University, Ramat Aviv 69978, Israel
Eilam, T., Inst. for Cereal Crop Improvement, Tel Aviv University, Ramat Aviv 69978, Israel
Anikster, Y., Inst. for Cereal Crop Improvement, Tel Aviv University, Ramat Aviv 69978, Israel
Tissue culture of the alternate hosts of wheat, rye and oat rusts and their response to in vitro inoculation with the rust pathogens
Alternate host plants of cereal rust fungi are necessary for studying the rust sexual cycle and pathogenicity. These plants are usually difficult to propagate through cloning, while seed-propagated plants may have variable responses to the pathogen. To overcome these obstacles, tissue culture, under controlled and aseptic conditions, was utilized for clonal propagation and in vitro inoculation of the following species: Rhamnus palaestinus Boiss., the alternate host of oat (Avena spp.) crown rust (Puccinia coronata Corda); Thalictrum speciosissimum L., the alternate host of brown leaf rust of wheat (Puccinia recondita f. sp. tritici Eriks. and Henn.); and Lycopsis arvensis L., the alternate host of rye (Secala spp.) leaf rust (Puccinia recondita f. sp. recondita Rob. and Desm.). Shoot culture procedures for initial establishment and proliferation were developed for all three alternate host species. Shoot cultures were multiplied at rates ranging from 0.3 to 1.7 shoots/week. Successful infection following inoculation with teliospores of the corresponding rust fungi was obtained for R. palaestinus and T. speciosissimum but not for L. arvensis. The hardening and acclimatization efficiency of rooted T. speciosissimum and L. arvensis was of 80-90%. The propagation efficiency for R. palaestinus was not successful because of the low rate and poor quality of its rooting. It is concluded that the in vitro system might be used as an alternative method for inoculation and multiplication of alternate hosts of cereal rusts, although more experimentation is needed to define accurately the appropriate conditions for the proper infection response.
Scientific Publication
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