Aroch, I., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, Israel
Arogeti, I., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, Israel
Marcovics, A., Department of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagan, Israel
Spiegel, Y., Division of Nematology, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan, Israel
Lavy, E., Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot, Israel

Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S. lupi nematodes at different life stages, the L2 and L3 larvae (dead and alive) and to dead adult worms, with negative controls, with and without addition of the six respective inhibitory sugar haptens. Con A moderately bound to surfaces of both live and frozen L3, to the stoma and excretory pores of adult worms, and to the outer surface nematode's eggs, within a female worm, but not to L2. PNA bound only to stoma and excretory pores surfaces in both frozen and live L3. WGA bound strongly to the outer surfaces of live and dead L2 and L3, which resulted in molting of live larvae. These results suggest that the nematode's surface content change during its development. Such changes may play roles in the nematode's interactions with the intermediate and definitive hosts’ tissues, and in its ability to evade the immune response, its long survival within the host, and even induce neoplastic transformation. © 2017 Elsevier B.V.