אסיף מאגר המחקר החקלאי

Species of RNA extracted from tobacco and Datura plants and their differential sensitivity to actinomycin D

Year:

1971

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Volume :

44

Co-Authors:

Antignus, Y., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, Israel
Sela, I., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, Israel
Harpaz, I., Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot, Israel

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Abstract:

RNA was extracted from leaves of tobacco with phenol and sodium codecyl sulfate (SDS) and analyzed on sucrose gradients. A 13s RNA component was found to be associated with the light ribosomal RNA peak, shifting this peak towards the 13s region. The synthesis of this 13s RNA species is relatively resistant to actinomycin D, and is almost absent from RNA preparations extracted without SDS. A similar peak was found in sucrose gradient profiles of Datura stramonium RNA, but at the 11s region of the gradient. RNA synthesis in Datura leaves is blocked following detachment of the leaves from the plant, but the synthesis of this particular 11s RNA component does not cease, either in detached leaves or in actinomycin-treated leaves. The RNA nature of this AMD-resistant peak was established by its sensitivity to ribonuclease, by its insensitivity to deoxyribonuclease, as well as by electrophoresis and radioactive scanning of polyacrylamide gels. © 1971.

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