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Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts
Year:
1980
Source of publication :
Journal of Clinical Microbiology
Authors :
Eshdat, Yuval
;
.
Volume :
11
Co-Authors:
Mirelman, D., Dept. Biophys., Weizmann Inst. Sci., Rohovoth, Israel
Altmann, G., Dept. Biophys., Weizmann Inst. Sci., Rohovoth, Israel
Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rohovoth, Israel
Facilitators :
From page:
328
To page:
331
(
Total pages:
4
)
Abstract:
A total of 393 clinical bacterial isolates were tested for their ability to agglutinate yeast cells of either Saccharomyces cerevisiae or Candida albicans. A positive agglutination of yeasts that could be prevented by methyl α-D-mannoside was taken as an indication for the possible presence of a mannose-specific lectin (carbohydrate-binding protein) on the surface of the tested bacteria. Agglutination tests on glass slides showed that 38% of all the isolates tested were positive in their capacity to agglutinate yeasts. Among the various strains tested, all isolates of Serratia marcescens, Proteus morganii, and Citrobacter diversus, as well as 94% of Klebsiella pneumoniae, were positive. On the other hand, only 46% of the Escherichia coli, 48% of the salmonellae, 44% of the Citrobacter freundii, and 71% of the Aeromonas hydrophila isolates were positive. A quantitative determination of the lectin activity done by observing the agglutination of yeasts in microtiter plates showed that S. marcescens isolates were the most avid binders of the yeast, whereas Klebsiella and Citrobacter isolates were the weakest.
Note:
Related Files :
Agglutination
antigen antibody reaction
bacteria
Biological Assay
Candida albicans
Enterobacteriaceae
lectins
Saccharomyces cerevisiae
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
22729
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:54
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Scientific Publication
Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts
11
Mirelman, D., Dept. Biophys., Weizmann Inst. Sci., Rohovoth, Israel
Altmann, G., Dept. Biophys., Weizmann Inst. Sci., Rohovoth, Israel
Eshdat, Y., Dept. Biophys., Weizmann Inst. Sci., Rohovoth, Israel
Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts
A total of 393 clinical bacterial isolates were tested for their ability to agglutinate yeast cells of either Saccharomyces cerevisiae or Candida albicans. A positive agglutination of yeasts that could be prevented by methyl α-D-mannoside was taken as an indication for the possible presence of a mannose-specific lectin (carbohydrate-binding protein) on the surface of the tested bacteria. Agglutination tests on glass slides showed that 38% of all the isolates tested were positive in their capacity to agglutinate yeasts. Among the various strains tested, all isolates of Serratia marcescens, Proteus morganii, and Citrobacter diversus, as well as 94% of Klebsiella pneumoniae, were positive. On the other hand, only 46% of the Escherichia coli, 48% of the salmonellae, 44% of the Citrobacter freundii, and 71% of the Aeromonas hydrophila isolates were positive. A quantitative determination of the lectin activity done by observing the agglutination of yeasts in microtiter plates showed that S. marcescens isolates were the most avid binders of the yeast, whereas Klebsiella and Citrobacter isolates were the weakest.
Scientific Publication
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