נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Independent segregation of the plastid genome and cytoplasmic male sterility in Petunia somatic hybrids
Year:
1985
Source of publication :
MGG Molecular & General Genetics
Authors :
Clark, Ellen M.
;
.
Izhar, Shamay
;
.
Volume :
199
Co-Authors:
Clark, E.M., Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, 22903, VA, United States
Izhar, S., Department of Plant Genetics and Breeding, Volcani Center, ARO, Bet Dagan, 50-250, Israel
Hanson, M.R., Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, 22903, VA, United States
Facilitators :
From page:
440
To page:
445
(
Total pages:
6
)
Abstract:
The chloroplast genomes of three sets of Petunia somatic hybrids were analyzed to examine the relationship between chloroplast DNA (cpDNA) composition and cytoplasmic male sterility (CMS). Chloroplast genomes of somatic hybrid plants were identified either by restriction and electrophoresis of purified cpDNAs or by hybridization of total DNA digests with cloned cpDNA probes that distinguish the parental genomes. The chloroplast genomes of a set of seven somatic hybrids derived from the fusion of Petunia CMS line 2423 and fertile line 3699 were analyzed. All seven plants were fertile, and all exhibited the cpDNA restriction pattern of the sterile cytoplasm. Similarly, four fertile somatic hybrids derived from the fusion of CMS line 3688 and fertile line 3677 were found to contain the CMS chloroplast genome. The cpDNA compositions of four fertile and two sterile somatic hybrids derived from the fusion of CMS line 3688 and fertile line 3704 were determined by restriction analysis of purified cpDNAs; all six plants exhibited the cpDNA restriction pattern of line 3704. Thus the CMS phenotype segregates independently of the chloroplast genome in Petunia somatic hybrids, indicating that CMS in Petunia is not specified by the chloroplast genome. © 1985 Springer-Verlag.
Note:
Related Files :
Show More
Related Content
More details
DOI :
10.1007/BF00330756
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
22871
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:55
You may also be interested in
Scientific Publication
Independent segregation of the plastid genome and cytoplasmic male sterility in Petunia somatic hybrids
199
Clark, E.M., Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, 22903, VA, United States
Izhar, S., Department of Plant Genetics and Breeding, Volcani Center, ARO, Bet Dagan, 50-250, Israel
Hanson, M.R., Department of Biology, University of Virginia, Gilmer Hall, Charlottesville, 22903, VA, United States
Independent segregation of the plastid genome and cytoplasmic male sterility in Petunia somatic hybrids
The chloroplast genomes of three sets of Petunia somatic hybrids were analyzed to examine the relationship between chloroplast DNA (cpDNA) composition and cytoplasmic male sterility (CMS). Chloroplast genomes of somatic hybrid plants were identified either by restriction and electrophoresis of purified cpDNAs or by hybridization of total DNA digests with cloned cpDNA probes that distinguish the parental genomes. The chloroplast genomes of a set of seven somatic hybrids derived from the fusion of Petunia CMS line 2423 and fertile line 3699 were analyzed. All seven plants were fertile, and all exhibited the cpDNA restriction pattern of the sterile cytoplasm. Similarly, four fertile somatic hybrids derived from the fusion of CMS line 3688 and fertile line 3677 were found to contain the CMS chloroplast genome. The cpDNA compositions of four fertile and two sterile somatic hybrids derived from the fusion of CMS line 3688 and fertile line 3704 were determined by restriction analysis of purified cpDNAs; all six plants exhibited the cpDNA restriction pattern of line 3704. Thus the CMS phenotype segregates independently of the chloroplast genome in Petunia somatic hybrids, indicating that CMS in Petunia is not specified by the chloroplast genome. © 1985 Springer-Verlag.
Scientific Publication
You may also be interested in