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The rate of mitochondrial protein synthesis during synchronized division of Astasia
Year:
1967
Source of publication :
Biochemistry (source )
Authors :
Kahn, Varda
;
.
Volume :
6
Co-Authors:
Kahn, V.
Blum, J.J., Department of Physiology and Pharmacology, Duke University, Durham, NC, United States
Facilitators :
From page:
817
To page:
826
(
Total pages:
10
)
Abstract:
Cultures of Astasia longa were synchronized by a repetitive temperature cycle. At various times of the cycle, cells were pulse labeled with 35SO4 for 30 min, then rapidly chilled, and collected. A portion of the cells was used for determining the specific activity of the sulfur amino acids in the trichloroacetic acid soluble pool. About 85% of the radioactivity in the trichloroacetic acid soluble pool was in cysteine, the remainder being mostly in methionine. The specific activity of the cysteine pool at the end of a 30-min pulse increased throughout the warm period, reaching a level of about three times that obtained during a pulse in the cold period. Mitochondria were prepared from the remainder of the cells and purified by isopycnic sucrose gradient sedimentation. The purified mitochondrial fraction was extracted with trichloroacetic acid. Over 95% of the radioactivity in the tri-chloroacetic acid insoluble pellet was present as protein. Amino acid analysis of this pellet showed no significant change in the sulfur amino acid composition of mitochondrial protein during the warm period of the cell cycle. From the measured specific activities of the mitochondrial protein and of the sulfur amino acid pool, the amount of newly synthesized mitochondrial protein per unit mitochondrial protein was computed for each 30-min pulse period. The results showed that the rate of mitochondrial protein synthesis was highest early in the warm period (i.e., before cell division), decreased as the warm period proceeded, and continues throughout the cold period. The data, however, do not indicate any significant change in the ratio of mitochondrial protein synthesis rate to cell protein synthesis rate at any time of the cell cycle.
Note:
Related Files :
biosynthesis
Cell Division
Mastigophora
metabolism
proteins
sulfate
Sulfates
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
22958
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:55
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Scientific Publication
The rate of mitochondrial protein synthesis during synchronized division of Astasia
6
Kahn, V.
Blum, J.J., Department of Physiology and Pharmacology, Duke University, Durham, NC, United States
The rate of mitochondrial protein synthesis during synchronized division of Astasia
Cultures of Astasia longa were synchronized by a repetitive temperature cycle. At various times of the cycle, cells were pulse labeled with 35SO4 for 30 min, then rapidly chilled, and collected. A portion of the cells was used for determining the specific activity of the sulfur amino acids in the trichloroacetic acid soluble pool. About 85% of the radioactivity in the trichloroacetic acid soluble pool was in cysteine, the remainder being mostly in methionine. The specific activity of the cysteine pool at the end of a 30-min pulse increased throughout the warm period, reaching a level of about three times that obtained during a pulse in the cold period. Mitochondria were prepared from the remainder of the cells and purified by isopycnic sucrose gradient sedimentation. The purified mitochondrial fraction was extracted with trichloroacetic acid. Over 95% of the radioactivity in the tri-chloroacetic acid insoluble pellet was present as protein. Amino acid analysis of this pellet showed no significant change in the sulfur amino acid composition of mitochondrial protein during the warm period of the cell cycle. From the measured specific activities of the mitochondrial protein and of the sulfur amino acid pool, the amount of newly synthesized mitochondrial protein per unit mitochondrial protein was computed for each 30-min pulse period. The results showed that the rate of mitochondrial protein synthesis was highest early in the warm period (i.e., before cell division), decreased as the warm period proceeded, and continues throughout the cold period. The data, however, do not indicate any significant change in the ratio of mitochondrial protein synthesis rate to cell protein synthesis rate at any time of the cell cycle.
Scientific Publication
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