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Cyclic AMP-dependent inhibition of collagen synthesis in avian epiphyseal cartilage cells: effect of chicken and human parathyroid hormone and parathyroid hormone-related peptide
Year:
1990
Source of publication :
Bone and Mineral
Authors :
Granot, Irit
;
.
Hurvitz, Shmuel (Animal science)
;
.
Pines, Mark
;
.
Volume :
9
Co-Authors:
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Granot, I., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Hurwitz, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Facilitators :
From page:
23
To page:
33
(
Total pages:
11
)
Abstract:
Avian cartilage cells derived from epiphyseal growth-plate and avian skin fibroblasts were cultured in vitro. Production of cAMP by cartilage cells was stimulated by the synthetic fragments (l-34) of chicken (cPTH), human (hPTH) parathyroid hormone and by parathyroid hormone-related peptide (PTHrP). The enhancement of cAMP production by any of the peptides could be blocked by the parathyroid hormone analogue (3-34)PTH, suggesting interaction with PTH specific receptors. When incubated with [3H]proline, both cell types released radiolabelled collagenase-digestible and non-digestible proteins into the medium. cPTH, hPTH, PTHrP, forskolin, prostaglandin E2 (PGE2) and 8-bromo cAMP inhibited collagen production in cartilage cells with only minor effects on non-collagenase digestible proteins. No effect of cAMP on collagen production by fibroblasts was observed. The present results provide additional evidence that avian growth-plate cartilage cells are targets for PTH, and are first to demonstrate the response of a non-mammalian system to mammalian PTHrP. The data suggest that collagen production by epiphyseal growth-plate cartilage cells is inhibited by PTH and that this inhibition is mediated by cAMP. © 1990.
Note:
Related Files :
Animal
Chickens
Cyclic AMP
Growth plate
Mammalia
proteins
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More details
DOI :
10.1016/0169-6009(90)90097-Y
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
22978
Last updated date:
21/08/2022 07:45
Creation date:
16/04/2018 23:55
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Scientific Publication
Cyclic AMP-dependent inhibition of collagen synthesis in avian epiphyseal cartilage cells: effect of chicken and human parathyroid hormone and parathyroid hormone-related peptide
9
Pines, M., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Granot, I., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Hurwitz, S., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel
Cyclic AMP-dependent inhibition of collagen synthesis in avian epiphyseal cartilage cells: effect of chicken and human parathyroid hormone and parathyroid hormone-related peptide
Avian cartilage cells derived from epiphyseal growth-plate and avian skin fibroblasts were cultured in vitro. Production of cAMP by cartilage cells was stimulated by the synthetic fragments (l-34) of chicken (cPTH), human (hPTH) parathyroid hormone and by parathyroid hormone-related peptide (PTHrP). The enhancement of cAMP production by any of the peptides could be blocked by the parathyroid hormone analogue (3-34)PTH, suggesting interaction with PTH specific receptors. When incubated with [3H]proline, both cell types released radiolabelled collagenase-digestible and non-digestible proteins into the medium. cPTH, hPTH, PTHrP, forskolin, prostaglandin E2 (PGE2) and 8-bromo cAMP inhibited collagen production in cartilage cells with only minor effects on non-collagenase digestible proteins. No effect of cAMP on collagen production by fibroblasts was observed. The present results provide additional evidence that avian growth-plate cartilage cells are targets for PTH, and are first to demonstrate the response of a non-mammalian system to mammalian PTHrP. The data suggest that collagen production by epiphyseal growth-plate cartilage cells is inhibited by PTH and that this inhibition is mediated by cAMP. © 1990.
Scientific Publication
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