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The involvement of a vanadate‐sensitive ATPase in plasma membranes of a salt tolerant cyanobacterium
Year:
1994
Source of publication :
Physiologia Plantarum
Authors :
Ben Hayyim, Gosal
;
.
Volume :
90
Co-Authors:
Gabbay‐Azaria, R., Dept of Genetics, Life Science Institute., Hebrew Univ. of Jerusalem, Jerusalem, 91904, Israel
Pick, U., Dept of Biochemistry, Weizmann Institute of Science, Rehovot, 76100, Israel
Ben‐Hayyim, G., Institute of Horticulture, ARO, Bet-Dagan, Israel
Tel‐Or, E., Dept of Agricultural Botany, Faculty of Agriculture, Hebrew Univ. of Jerusalem, Rehovot, 76100, Israel
Facilitators :
From page:
692
To page:
698
(
Total pages:
7
)
Abstract:
Plasma membranes of the marine cyanobacterium Spirulina subsalsa were tested for ATPase activity, and for involvement in salt stress. Transition of cells from saline to hypersaline medium enhances the respiratory activity associated with extrusion of Na+ and Cl−, and persisting salt stress induces synthesis of respiratory enzymes in the plasma membranes. The membranes possess an ATPase, specific for ATP and Mg2+ and sensitive to orthovanadate and dicyclohexylcarbodiimide. Immunoblot analysis of plasma membrane polypeptides from Spirulina subsalsa with anti‐Arabidopsis H+‐ATPase serum identified a single polypeptide of 100 kDa, which cross‐reacted with the antibodies. An unusual feature of this ATPase is a specific stimulation by Na+ ions. Prolonged adaptation of S. subsals cells to hypersaline conditions induced an increase in ATPase activity in subsequent plasma membrane preparations, as well as a higher content of the 100 kDa polypeptide. It is suggested that the ATPase investigated is an H+‐pump, which is involved in extrusion of Na+ and in conferring resistance to salt stress. Copyright © 1994, Wiley Blackwell. All rights reserved
Note:
Related Files :
Cyanobacterial plasma membranes
H+‐pumps
Na+‐extrusion
respiration
Spirulina subsalsa
Show More
Related Content
More details
DOI :
10.1111/j.1399-3054.1994.tb02525.x
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23109
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:56
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Scientific Publication
The involvement of a vanadate‐sensitive ATPase in plasma membranes of a salt tolerant cyanobacterium
90
Gabbay‐Azaria, R., Dept of Genetics, Life Science Institute., Hebrew Univ. of Jerusalem, Jerusalem, 91904, Israel
Pick, U., Dept of Biochemistry, Weizmann Institute of Science, Rehovot, 76100, Israel
Ben‐Hayyim, G., Institute of Horticulture, ARO, Bet-Dagan, Israel
Tel‐Or, E., Dept of Agricultural Botany, Faculty of Agriculture, Hebrew Univ. of Jerusalem, Rehovot, 76100, Israel
The involvement of a vanadate‐sensitive ATPase in plasma membranes of a salt tolerant cyanobacterium
Plasma membranes of the marine cyanobacterium Spirulina subsalsa were tested for ATPase activity, and for involvement in salt stress. Transition of cells from saline to hypersaline medium enhances the respiratory activity associated with extrusion of Na+ and Cl−, and persisting salt stress induces synthesis of respiratory enzymes in the plasma membranes. The membranes possess an ATPase, specific for ATP and Mg2+ and sensitive to orthovanadate and dicyclohexylcarbodiimide. Immunoblot analysis of plasma membrane polypeptides from Spirulina subsalsa with anti‐Arabidopsis H+‐ATPase serum identified a single polypeptide of 100 kDa, which cross‐reacted with the antibodies. An unusual feature of this ATPase is a specific stimulation by Na+ ions. Prolonged adaptation of S. subsals cells to hypersaline conditions induced an increase in ATPase activity in subsequent plasma membrane preparations, as well as a higher content of the 100 kDa polypeptide. It is suggested that the ATPase investigated is an H+‐pump, which is involved in extrusion of Na+ and in conferring resistance to salt stress. Copyright © 1994, Wiley Blackwell. All rights reserved
Scientific Publication
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