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Fermentative metabolism in grape berries: Isolation and characterization of pyruvate decarboxylase cDNA and analysis of its expression throughout berry development
Year:
2000
Source of publication :
Plant Science
Authors :
Ogrodovitch, Aliza
;
.
Or, Etti
;
.
Sadka, Avi
;
.
Volume :
156
Co-Authors:
Or, E., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Baybik, J., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Sadka, A., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Ogrodovitch, A., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Facilitators :
From page:
151
To page:
158
(
Total pages:
8
)
Abstract:
The involvement of pyruvate decarboxylase (PDC) in the control of alcohol production during ripening of fruit tissues under aerobic conditions has been only partially studied. Enzymological studies showed a significant increase in PDC activity during the ripening of oranges and pears, concurrently with the induction of ethanol production. In tomato, on the other hand, the induction of ethanol production and ADH gene expression after the onset of ripening was not accompanied by induction of PDC activity. The isolation of PDC cDNA from fruits has not yet been reported, nor has its expression pattern during fruit development. We report here the isolation of a cDNA clone encoding for a grape PDC and the characterization of its expression throughout berry development. The pattern of PDC gene expression throughout berry development, combined with earlier findings on constitutive PDC activity in the berry, may suggest that PDC is not the limiting factor for the production of ethanol in the berry, which is induced only after the onset of berry ripening. Alternatively, the induction of ADH gene expression, which occurs only after the onset of ripening in both tomatoes and grape berries, may serve as a regulator of ethanol production in response to a ripening-related cue. (C) 2000 Elsevier Science Ireland Ltd.
Note:
Related Files :
alcohol
article
enzyme analysis
enzyme isolation
Fermentation
grape
nucleotide sequence
plant growth
Show More
Related Content
More details
DOI :
10.1016/S0168-9452(00)00247-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23195
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:57
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Scientific Publication
Fermentative metabolism in grape berries: Isolation and characterization of pyruvate decarboxylase cDNA and analysis of its expression throughout berry development
156
Or, E., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Baybik, J., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Sadka, A., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Ogrodovitch, A., Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50-250, Israel
Fermentative metabolism in grape berries: Isolation and characterization of pyruvate decarboxylase cDNA and analysis of its expression throughout berry development
The involvement of pyruvate decarboxylase (PDC) in the control of alcohol production during ripening of fruit tissues under aerobic conditions has been only partially studied. Enzymological studies showed a significant increase in PDC activity during the ripening of oranges and pears, concurrently with the induction of ethanol production. In tomato, on the other hand, the induction of ethanol production and ADH gene expression after the onset of ripening was not accompanied by induction of PDC activity. The isolation of PDC cDNA from fruits has not yet been reported, nor has its expression pattern during fruit development. We report here the isolation of a cDNA clone encoding for a grape PDC and the characterization of its expression throughout berry development. The pattern of PDC gene expression throughout berry development, combined with earlier findings on constitutive PDC activity in the berry, may suggest that PDC is not the limiting factor for the production of ethanol in the berry, which is induced only after the onset of berry ripening. Alternatively, the induction of ADH gene expression, which occurs only after the onset of ripening in both tomatoes and grape berries, may serve as a regulator of ethanol production in response to a ripening-related cue. (C) 2000 Elsevier Science Ireland Ltd.
Scientific Publication
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