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Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining
Year:
2013
Source of publication :
Journal of Microbiological Methods
Authors :
Bernstein, Nirit
;
.
Volume :
94
Co-Authors:
Orlofsky, E., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990, Israel
Gillor, O., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990, Israel
Melli, A., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA 95616, United States
Miller, W., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA 95616, United States
Wuertz, S., Department of Civil and Environmental Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States, Singapore Centre on Environmental Life Sciences Engineering, School of Biological Sciences SBS-B1N-27 and School of Civil and Environmental Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore
Bernstein, N., Institute of Soil Water and Environmental Sciences, Volcani Center, POB 6, Bet-Dagan, 50-250, Israel
Shapiro, K., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA 95616, United States
Facilitators :
From page:
375
To page:
377
(
Total pages:
3
)
Abstract:
An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g-1 soil. © 2013 Elsevier B.V.
Note:
Related Files :
concentration (parameters)
Immunomagnetic separation
process optimization
Recovery
soil
suspension
Show More
Related Content
More details
DOI :
10.1016/j.mimet.2013.07.019
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23284
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:58
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Scientific Publication
Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining
94
Orlofsky, E., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990, Israel
Gillor, O., Zuckerberg Institute for Water Research, J. Blaustein Institutes for Desert Research, Ben-Gurion University, Midreshet Ben Gurion, 84990, Israel
Melli, A., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA 95616, United States
Miller, W., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA 95616, United States
Wuertz, S., Department of Civil and Environmental Engineering, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States, Singapore Centre on Environmental Life Sciences Engineering, School of Biological Sciences SBS-B1N-27 and School of Civil and Environmental Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore
Bernstein, N., Institute of Soil Water and Environmental Sciences, Volcani Center, POB 6, Bet-Dagan, 50-250, Israel
Shapiro, K., Department of Pathology, Microbiology and Immunology, University of California, One Shields Avenue, Davis, CA 95616, United States
Simultaneous detection of Giardia lamblia and Cryptosporidium parvum (oo)cysts in soil using immunomagnetic separation and direct fluorescent antibody staining
An improved approach for simultaneous detection of Cryptosporidium parvum and Giardia lamblia (oo)cysts in soil is described. Recoveries>70% were obtained for concentrations>55 and 21 (oo)cysts g-1 for C. parvum and G. lamblia, respectively. The limits of detection were determined to be<5 (oo)cysts g-1 soil. © 2013 Elsevier B.V.
Scientific Publication
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