נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Induced diploid gynogenesis in white bass
Year:
1998
Source of publication :
Progressive Fish-Culturist
Authors :
Cherfas, Nina B.
;
.
Hulata, Gideon
;
.
Volume :
60
Co-Authors:
Gomelsky, B., Min. of Agric. and Rural Development, Department of Fisheries Fish, Aquaculture Research Station, Dor, M. P. Hof Hacarmel, 30820, Israel, Aquaculture Research Center, Kentucky State University, Frankfort, KY 40601, United States
Cherfas, N.B., Agricultural Research Organization, Institute of Animal Science, Department of Aquaculture, Dor, M. P. Hof Hacarmel, 30820, Israel
Gissis, A., Fish Hatchery, Kibbutz Hamaapil, Israel
Hulata, G., Agricultural Research Organization, Institute of Animal Science, Department of Aquaculture, Post Office Box 6, Bet Dagan, 50250, Israel
Facilitators :
From page:
288
To page:
292
(
Total pages:
5
)
Abstract:
Optimal conditions were determined for induction of meiotic diploid gynogenesis in white bass Morone chrysops. In two series of experiments, ultraviolet light (UV) irradiation doses to inactivate sperm genome and optimal temperature shock to restore diploidy were established. Eggs of white bass were inseminated with sperm from striped bass M. saxatilis, diluted (1:30) in saline and irradiated with UV at doses ranging from 50 to 1,200 J/m2. A typical 'Hertwig effect' was observed with increasing UV dose. Larvae obtained at doses of 400 J/m2 and greater were abnormally developed (haploid syndrome) and had a haploid chromosome number (n = 24). Heat shocks to cause retention of the second polar body were applied. Heat shocks of 2-min duration at 36°C, 38°C, or 40°C were applied at 2 or 3 min after insemination to white bass eggs inseminated with irradiated (800 J/m2) striped bass sperm. The best results were obtained after application of 36°C heat shock at 3 min after insemination, which induced a significant increase in yield of diploid gynogens (24-39%, from initial numbers of gynogenetic embryos) and provided relatively high postshock embryo survival. The gynogenetic origin of diploids obtained was confirmed by the lack of melanophores in putative gynogens (contrary to pigmented control hybrid larvae).
Note:
Related Files :
artificial insemination
chromosome number
dilution
diploidy
Gynogenesis
meiosis
Morone saxatilis
ultraviolet radiation
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23334
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:58
You may also be interested in
Scientific Publication
Induced diploid gynogenesis in white bass
60
Gomelsky, B., Min. of Agric. and Rural Development, Department of Fisheries Fish, Aquaculture Research Station, Dor, M. P. Hof Hacarmel, 30820, Israel, Aquaculture Research Center, Kentucky State University, Frankfort, KY 40601, United States
Cherfas, N.B., Agricultural Research Organization, Institute of Animal Science, Department of Aquaculture, Dor, M. P. Hof Hacarmel, 30820, Israel
Gissis, A., Fish Hatchery, Kibbutz Hamaapil, Israel
Hulata, G., Agricultural Research Organization, Institute of Animal Science, Department of Aquaculture, Post Office Box 6, Bet Dagan, 50250, Israel
Induced diploid gynogenesis in white bass
Optimal conditions were determined for induction of meiotic diploid gynogenesis in white bass Morone chrysops. In two series of experiments, ultraviolet light (UV) irradiation doses to inactivate sperm genome and optimal temperature shock to restore diploidy were established. Eggs of white bass were inseminated with sperm from striped bass M. saxatilis, diluted (1:30) in saline and irradiated with UV at doses ranging from 50 to 1,200 J/m2. A typical 'Hertwig effect' was observed with increasing UV dose. Larvae obtained at doses of 400 J/m2 and greater were abnormally developed (haploid syndrome) and had a haploid chromosome number (n = 24). Heat shocks to cause retention of the second polar body were applied. Heat shocks of 2-min duration at 36°C, 38°C, or 40°C were applied at 2 or 3 min after insemination to white bass eggs inseminated with irradiated (800 J/m2) striped bass sperm. The best results were obtained after application of 36°C heat shock at 3 min after insemination, which induced a significant increase in yield of diploid gynogens (24-39%, from initial numbers of gynogenetic embryos) and provided relatively high postshock embryo survival. The gynogenetic origin of diploids obtained was confirmed by the lack of melanophores in putative gynogens (contrary to pigmented control hybrid larvae).
Scientific Publication
You may also be interested in