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Chen, S., Department of Food Science, ARO, Volcani Center, Bet-Dagan, Israel
Glazer, I., Department of Nematology, ARO, Volcani Center, Bet-Dagan, Israel
Gollop, N., Department of Food Science, ARO, Volcani Center, Bet-Dagan, Israel
Cash, P., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Argo, E., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Innes, A., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Stewart, E., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Davidson, I., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Wilson, M.J., School of Biological Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
In order to improve the storage capability under desiccation of the widely sold biological insecticides based on entomopathogenic nematodes (EPNs), we need to understand how these organisms respond to desiccation stress. As part of our studies to achieve this, we studied survival and protein expression in infective juveniles of the EPN Steinernema feltiae IS-6 when exposed to evaporative (exposure to 97% relative humidity (RH) for 3 days, followed by a 1-day exposure to 85% RH) and osmotic (exposure to 24% glycerol for 8 h) stresses. More than 400 protein spots that were detected by proteomic analysis showed reproducible abundance within replications. Of these, 10 spots and 7 spots showed detectable changes in abundance under evaporative and osmotic stress, respectively, compared to fully hydrated nematodes. Three spots exhibited a differential response pattern between evaporative and osmotic desiccation (one was down regulated and two were novel in evaporative desiccation). Peptide mass mapping with MALDI-TOF mass spectrometry (MS) identified 10 desiccation-response proteins, among which several are known to be stress responsive including heat shock protein 60, coenzyme q biosynthesis protein, inositol monophosphatase and fumarate lyase that were found in both stresses. Other identified proteins are known to be involved in the cell cycle regulation, regulation of gene transcription, organization of macromolecular structure and some currently have no known functions. Our results suggest that it is unlikely that improvement of desiccation tolerance in EPNs can be achieved through genetic transformation and addition of single genes and that selective breeding could be the best approach to generate desiccation resistant worms. © 2005 Elsevier B.V. All rights reserved.
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Proteomic analysis of the entomopathogenic nematode Steinernema feltiae IS-6 IJs under evaporative and osmotic stresses
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Chen, S., Department of Food Science, ARO, Volcani Center, Bet-Dagan, Israel
Glazer, I., Department of Nematology, ARO, Volcani Center, Bet-Dagan, Israel
Gollop, N., Department of Food Science, ARO, Volcani Center, Bet-Dagan, Israel
Cash, P., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Argo, E., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Innes, A., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Stewart, E., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Davidson, I., Proteome Facility, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Wilson, M.J., School of Biological Sciences, University of Aberdeen, Aberdeen AB24 3UU, United Kingdom
Proteomic analysis of the entomopathogenic nematode Steinernema feltiae IS-6 IJs under evaporative and osmotic stresses
In order to improve the storage capability under desiccation of the widely sold biological insecticides based on entomopathogenic nematodes (EPNs), we need to understand how these organisms respond to desiccation stress. As part of our studies to achieve this, we studied survival and protein expression in infective juveniles of the EPN Steinernema feltiae IS-6 when exposed to evaporative (exposure to 97% relative humidity (RH) for 3 days, followed by a 1-day exposure to 85% RH) and osmotic (exposure to 24% glycerol for 8 h) stresses. More than 400 protein spots that were detected by proteomic analysis showed reproducible abundance within replications. Of these, 10 spots and 7 spots showed detectable changes in abundance under evaporative and osmotic stress, respectively, compared to fully hydrated nematodes. Three spots exhibited a differential response pattern between evaporative and osmotic desiccation (one was down regulated and two were novel in evaporative desiccation). Peptide mass mapping with MALDI-TOF mass spectrometry (MS) identified 10 desiccation-response proteins, among which several are known to be stress responsive including heat shock protein 60, coenzyme q biosynthesis protein, inositol monophosphatase and fumarate lyase that were found in both stresses. Other identified proteins are known to be involved in the cell cycle regulation, regulation of gene transcription, organization of macromolecular structure and some currently have no known functions. Our results suggest that it is unlikely that improvement of desiccation tolerance in EPNs can be achieved through genetic transformation and addition of single genes and that selective breeding could be the best approach to generate desiccation resistant worms. © 2005 Elsevier B.V. All rights reserved.
Scientific Publication
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