Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Cryopreservation of in vitro-grown shoot tips of grapevine by encapsulation-dehydration
Year:
2000
Authors :
Gafny, Ron
;
.
Volume :
63
Co-Authors:
Wang, Q., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Tanne, E., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gafny, R., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
41
To page:
46
(
Total pages:
6
)
Abstract:
In vitro-grown shoot tips of the LN33 hybrid (Vitis L.) and cv. Superior (Vitis vinifera L.) were successfully cryopreserved by encapsulation-dehydration. Encapsulated shoot tips were precultured stepwise on half-strength MS medium supplemented with increasing sucrose concentrations of 0.25, 0.5, 0.75 and 1.0 M for 4 days, with one day for each step. Following preculture, encapsulated shoot tips were dehydrated prior to direct immersion in liquid nitrogen for 1 h. After thawing, cryopreserved shoot tips were post-cultured on a post-culture medium for survival. An optimal survival of cryopreserved shoot tips was achieved when encapsulated shoot tips were dehydrated to 15.6 and 17.6% water content for the LN33 hybrid and cv. Superior, respectively. Comparison between the effects of dehydration with silica gel and by air drying on cryopreserved shoot tips, showed that survival was dependent on water content, not on dehydration method. The thawing method markedly affected survival of cryopreserved shoot tips, and thawing at 40 °C for 3 min was found best. No callus formation and fastest shoot elongation were obtained when cryopreserved shoot tips were post-cultured on the post-culture medium composed of half-strength MS supplemented with 1 mg 1-1 BA and 0.1 mg 1-1 NAA. With these optimized parameters, 60 and 40% survival of cryopreserved shoot tips were obtained for the LN33 hybrid and cv. Superior, respectively.
Note:
Related Files :
6 n benzyladenine
concentration (parameters)
drying
Shoot apex
temperature
Vitis
woody plant
Show More
Related Content
More details
DOI :
10.1023/A:1006411829738
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23694
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:01
You may also be interested in
Scientific Publication
Cryopreservation of in vitro-grown shoot tips of grapevine by encapsulation-dehydration
63
Wang, Q., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Tanne, E., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Gafny, R., Department of Virology, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Cryopreservation of in vitro-grown shoot tips of grapevine by encapsulation-dehydration
In vitro-grown shoot tips of the LN33 hybrid (Vitis L.) and cv. Superior (Vitis vinifera L.) were successfully cryopreserved by encapsulation-dehydration. Encapsulated shoot tips were precultured stepwise on half-strength MS medium supplemented with increasing sucrose concentrations of 0.25, 0.5, 0.75 and 1.0 M for 4 days, with one day for each step. Following preculture, encapsulated shoot tips were dehydrated prior to direct immersion in liquid nitrogen for 1 h. After thawing, cryopreserved shoot tips were post-cultured on a post-culture medium for survival. An optimal survival of cryopreserved shoot tips was achieved when encapsulated shoot tips were dehydrated to 15.6 and 17.6% water content for the LN33 hybrid and cv. Superior, respectively. Comparison between the effects of dehydration with silica gel and by air drying on cryopreserved shoot tips, showed that survival was dependent on water content, not on dehydration method. The thawing method markedly affected survival of cryopreserved shoot tips, and thawing at 40 °C for 3 min was found best. No callus formation and fastest shoot elongation were obtained when cryopreserved shoot tips were post-cultured on the post-culture medium composed of half-strength MS supplemented with 1 mg 1-1 BA and 0.1 mg 1-1 NAA. With these optimized parameters, 60 and 40% survival of cryopreserved shoot tips were obtained for the LN33 hybrid and cv. Superior, respectively.
Scientific Publication
נגישות
menu      
You may also be interested in