אסיף מאגר המחקר החקלאי

Synthesis and secretion of caseins by the mouse mammary gland: Production and characterization of new polyclonal antibodies

Year:

1995

Source of publication :

Authors :

Volume :

144

Co-Authors:

Barash, I., Institute of Animal Science, ARO, the Volcani Center, Bet Dagan, 50250, Israel
Faerman, A., Institute of Animal Science, ARO, the Volcani Center, Bet Dagan, 50250, Israel
Puzis, R., Institute of Animal Science, ARO, the Volcani Center, Bet Dagan, 50250, Israel
Peterson, D., Institute of Animal Science, ARO, the Volcani Center, Bet Dagan, 50250, Israel
Shani, M., Institute of Animal Science, ARO, the Volcani Center, Bet Dagan, 50250, Israel

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Abstract:

Polyclonal antibodies to mouse α- and β/γ-caseins were raised in rabbits. These antibodies display tissue- and species specificity as shown by immunoblotting. Immunohistochemical analyses demonstrate that both α- and β/γ-caseins were synthesized and secreted from virtually all lactating mammary epithelial cells, in a pattern very similar to that of the mouse α-lactalbumin. Residual amounts of caseins were located also in the apical surface of epithelial cells surrounding the ducal lumen of virgin mammary gland sections. In contrast to the significant level of γ-casein in the milk, the amount of this protein compared to α- or β-caseins was extremely low in medium conditioned for 24 h by mammary explants of mid-pregnant mice immediately after explantation or after 4 days. © 1995 Kluwer Academic Publishers.

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