Applied Spectroscopy
Obeidat, S., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Baolong, B., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Rayson, G.D., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Anderson, D.M., United States Department of Agriculture, Agriculture Research Service, Jornada Experimental Range, Las Cruces, NM 88003-0003, United States
Puscheck, A.D., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Landau, S.Y., Department of Natural Resources and Agronomy, The Volcani Centre, Institute of Plant Science, Bet Dagan 50250, Israel
Glasser, T., Department of Natural Resources and Agronomy, The Volcani Centre, Institute of Plant Science, Bet Dagan 50250, Israel
A portable luminescence spectrofluorometer weighing only 1.5 kg that uses multiple light emitting diodes (LEDs) as excitation sources was developed and evaluated. Excitation using a sequence of seven individual broad-band LED emission sources enabled the generation of excitation-emission spectra using a light weight (<1.5 kg) spectrometer. Limits of detection for rhodamine 6G, rhodamine B, and fluorescein were 2.9, 3.2, and 11.0 nM, respectively. Generation of excitation-emission matrices (EEMs) enabled the analysis of samples containing mixtures of rhodamine B and fluorescein. Buffered saline plant and animal feed extracts were also analyzed using this instrument. These samples included the woody plants Pistacia lentiscus (Evergreen pistache or Mastic) and Philyria latifolia, and the herbaceous species Medicago sativa (alfalfa), Trifolium spp. (clover), and a feed concentrate. Application of multi-way principal component analysis (MPCA) to the resulting three-dimensional data sets enabled discernment among these various diet constituents. © 2008 Society for Applied Spectroscopy.
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A multi-source portable light emitting diode spectrofluorometer
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Obeidat, S., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Baolong, B., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Rayson, G.D., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Anderson, D.M., United States Department of Agriculture, Agriculture Research Service, Jornada Experimental Range, Las Cruces, NM 88003-0003, United States
Puscheck, A.D., Box 30001 MSC 3C, Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003-0001, United States
Landau, S.Y., Department of Natural Resources and Agronomy, The Volcani Centre, Institute of Plant Science, Bet Dagan 50250, Israel
Glasser, T., Department of Natural Resources and Agronomy, The Volcani Centre, Institute of Plant Science, Bet Dagan 50250, Israel
A multi-source portable light emitting diode spectrofluorometer
A portable luminescence spectrofluorometer weighing only 1.5 kg that uses multiple light emitting diodes (LEDs) as excitation sources was developed and evaluated. Excitation using a sequence of seven individual broad-band LED emission sources enabled the generation of excitation-emission spectra using a light weight (<1.5 kg) spectrometer. Limits of detection for rhodamine 6G, rhodamine B, and fluorescein were 2.9, 3.2, and 11.0 nM, respectively. Generation of excitation-emission matrices (EEMs) enabled the analysis of samples containing mixtures of rhodamine B and fluorescein. Buffered saline plant and animal feed extracts were also analyzed using this instrument. These samples included the woody plants Pistacia lentiscus (Evergreen pistache or Mastic) and Philyria latifolia, and the herbaceous species Medicago sativa (alfalfa), Trifolium spp. (clover), and a feed concentrate. Application of multi-way principal component analysis (MPCA) to the resulting three-dimensional data sets enabled discernment among these various diet constituents. © 2008 Society for Applied Spectroscopy.
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