נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Mutation in csrR global regulator reduces Streptococcus pyogenes internalization
Year:
2000
Source of publication :
Microbial Pathogenesis
Authors :
Sela, Shlomo
;
.
Volume :
29
Co-Authors:
Jadoun, J., Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
Facilitators :
From page:
311
To page:
317
(
Total pages:
7
)
Abstract:
Transposon (Tn916) mutagenesis was employed to identify genes in group A streptococcus (GAS) that are involved in bacterial internalization by epithelial cells. One mutant displayed significantly reduced internalization efficiency and was therefore selected for further characterization. The mutant harbored a single Tn916 insertion in csr, a genetic locus encoding a two-component regulatory system. Mutations in csr were found to derepress hyaluronic acid (HA) capsule synthesis. Since capsule expression has been previously reported to interfere with internalization of GAS, it was possible that the transposon exerted its inhibitory effect either by derepression of capsule synthesis, or by another mechanism. To study the effect of the csr mutation on bacterial internalization, isogenic mutants deficient in either csrR, hasA or both were generated. The hasA mutant adhered to and internalized into HEp-2 cells significantly better than the parent and the csrR mutant strains. The internalization efficiency of the double mutant (csrR-/hasA-) was reduced by seven-fold compared to that of the hasA mutant. These findings suggest that csrR affects streptococcal entry by modulating capsule expression as well as by another, yet unknown, mechanism. (C) 2000 Academic Press.
Note:
Related Files :
Cell Adhesion
gene expression
HEp-2 cells
human cell
Mutagenesis
mutation
regulator gene
Streptococcus sp. 'group A'
Show More
Related Content
More details
DOI :
10.1006/mpat.2000.0392
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23827
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:02
You may also be interested in
Scientific Publication
Mutation in csrR global regulator reduces Streptococcus pyogenes internalization
29
Jadoun, J., Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
Sela, S., Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel
Mutation in csrR global regulator reduces Streptococcus pyogenes internalization
Transposon (Tn916) mutagenesis was employed to identify genes in group A streptococcus (GAS) that are involved in bacterial internalization by epithelial cells. One mutant displayed significantly reduced internalization efficiency and was therefore selected for further characterization. The mutant harbored a single Tn916 insertion in csr, a genetic locus encoding a two-component regulatory system. Mutations in csr were found to derepress hyaluronic acid (HA) capsule synthesis. Since capsule expression has been previously reported to interfere with internalization of GAS, it was possible that the transposon exerted its inhibitory effect either by derepression of capsule synthesis, or by another mechanism. To study the effect of the csr mutation on bacterial internalization, isogenic mutants deficient in either csrR, hasA or both were generated. The hasA mutant adhered to and internalized into HEp-2 cells significantly better than the parent and the csrR mutant strains. The internalization efficiency of the double mutant (csrR-/hasA-) was reduced by seven-fold compared to that of the hasA mutant. These findings suggest that csrR affects streptococcal entry by modulating capsule expression as well as by another, yet unknown, mechanism. (C) 2000 Academic Press.
Scientific Publication
You may also be interested in