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Species identification of soil-borne Orobanche seeds by DNA fingerprinting
Year:
1997
Authors :
Joel, Daniel M.
;
.
Katzir, Nurit
;
.
Portnoy, Vitaly H.
;
.
Volume :
58
Co-Authors:
Portnoy, V.H., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Katzir, N., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Joel, D.M., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Facilitators :
From page:
49
To page:
54
(
Total pages:
6
)
Abstract:
DNA was extracted from single soil-borne seeds of Orobanche ramosa L., O. crenata Forsk, O. cumana Loeffl, and O. aegyptiaca. Species identification was then possible by RAPD-PCR, based on specific DNA markers, and could be verified by Southern hybridization with specific probes. Seed identification was also possible for dead (tetrazolium-negative) seeds. This method is a powerful diagnostic tool for Orobanche classification in agricultural fields.
Note:
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More details
DOI :
10.1006/pest.1997.2281
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23844
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:02
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Scientific Publication
Species identification of soil-borne Orobanche seeds by DNA fingerprinting
58
Portnoy, V.H., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Katzir, N., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Joel, D.M., Newe-Ya'ar Research Center, Agricultural Research Organization, P.O. Box 1021, Ramat-Yishay, 30095, Israel
Species identification of soil-borne Orobanche seeds by DNA fingerprinting
DNA was extracted from single soil-borne seeds of Orobanche ramosa L., O. crenata Forsk, O. cumana Loeffl, and O. aegyptiaca. Species identification was then possible by RAPD-PCR, based on specific DNA markers, and could be verified by Southern hybridization with specific probes. Seed identification was also possible for dead (tetrazolium-negative) seeds. This method is a powerful diagnostic tool for Orobanche classification in agricultural fields.
Scientific Publication
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