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Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees
Year:
2006
Source of publication :
Virus Genes
Authors :
Bar-Joseph, Moshe
;
.
Cohen, Oded
;
.
Mawassi, Munir
;
.
Volume :
33
Co-Authors:
Cohen, O., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Batuman, O., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Stanbekova, G., Ajtkhozhin Institute of Molecular Biology and Biochemistry, 480012, Almaty, Kazakhstan
Sano, T., Department of Plant Pathology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
287
To page:
292
(
Total pages:
6
)
Abstract:
Infections with different viroid species are common among cultivated fruit trees and grapevines, and many old-clone citrus varieties contain up to five citrus viroids (CVds) within a single tree. This paper describes the construction of a CVd-Multiprobe consisting of full-length clones of Hop stunt viroid, Citrus exocortis viroid, Citrus bent leaf viroid and CVd-III. The CVd-Multiprobe was tested against RNA transcripts of the four viroids and RNA extracts from plants singly infected with CEVd or HSVd or multiply infected with different CVds. The viroids were effectively diagnosed with the DIG labeled CVd-Multiprobe when tested by Northern hybridization or dot blot analyses. The CVd-Multiprobe does not provide information on the specific viroid resulting in a positive signal. However, this should not be considered as a problem, since most citrus certification programs will discard budwood source trees infected with any of the known CVds. © Springer Science+Business Media, LLC 2006.
Note:
Related Files :
Citrus bent leaf viroid
Citrus viroid III
Overlapping PCR
sequence analysis
tree
unclassified drug
viroid
Show More
Related Content
More details
DOI :
10.1007/s11262-006-0067-7
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
23900
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:03
Scientific Publication
Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees
33
Cohen, O., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Batuman, O., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Stanbekova, G., Ajtkhozhin Institute of Molecular Biology and Biochemistry, 480012, Almaty, Kazakhstan
Sano, T., Department of Plant Pathology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan
Mawassi, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Bar-Joseph, M., S. Tolkowsky Laboratory, Department of Virology, Volcani Center, Bet Dagan, Israel
Construction of a multiprobe for the simultaneous detection of viroids infecting citrus trees
Infections with different viroid species are common among cultivated fruit trees and grapevines, and many old-clone citrus varieties contain up to five citrus viroids (CVds) within a single tree. This paper describes the construction of a CVd-Multiprobe consisting of full-length clones of Hop stunt viroid, Citrus exocortis viroid, Citrus bent leaf viroid and CVd-III. The CVd-Multiprobe was tested against RNA transcripts of the four viroids and RNA extracts from plants singly infected with CEVd or HSVd or multiply infected with different CVds. The viroids were effectively diagnosed with the DIG labeled CVd-Multiprobe when tested by Northern hybridization or dot blot analyses. The CVd-Multiprobe does not provide information on the specific viroid resulting in a positive signal. However, this should not be considered as a problem, since most citrus certification programs will discard budwood source trees infected with any of the known CVds. © Springer Science+Business Media, LLC 2006.
Scientific Publication
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