The methylation state of 2 muscle-specific genes: Restriction enzyme analysis did not detect a correlation with expression

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The methylation state of 2 muscle-specific genes: Restriction enzyme analysis did not detect a correlation with expression

Year: 

1984

Source of publication :

Nucleic Acids Research

Authors :

Shani, Moshe

.

Volume :

Co-Authors: 

Shani, M., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Admon, S., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
Yaffe, D., Department of Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel

From page: 

7225

To page: 

7234

(

Total pages: 

10

)

Link :

The methylation state of 2 muscle-specific genes: Restriction enzyme analysis did not detect a correlation with expression

Abstract: 

To examine the possible role of DNA methylation in the modulation of expression of genes involved in the differentiation of muscle cells, we compared the methylation state of a number of CpG sites in the rat skeletal muscle actin and myosin light chain 2 genes, in muscle and nonmuscle cells, and in proliferating myoblasts and differentiated myotubes of the myogenic cell line L8. No correlation was detected between the state of methylation of these sites and the expression of the two genes. Essentially the same pattern of DNA methylation was observed, in the sites examined, in DNA from muscle, kidney and stomach. In DNA extracted from cultures of proliferating mononucleated myoblasts, as well as from differentiated multinucleated fibers of the myogenic cell line L8, the two genes were more methylated than in other tissues. © 1984 IRL Press Ltd.