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A new approach for weed control in a cucurbit field employing an attenuated potyvirus-vector for herbicide resistance
Year:
2001
Source of publication :
Journal of Biotechnology
Authors :
Arazi, Tzahi
;
.
Gal-On, Amit
;
.
Volume :
92
Co-Authors:
Shiboleth, Y.M., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Arazi, T., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Wang, Y., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Facilitators :
From page:
37
To page:
46
(
Total pages:
10
)
Abstract:
Expression of bar, a phosphinothricin acetyltransferase, in plant tissues, leads to resistance of these plants to glufosinate ammonium based herbicides. We have created a bar expressing, attenuated zucchini yellow mosaic potyvirus-vector, AGII-Bar, to enable herbicide use in cucurbit fields. The parental vector, ZYMV-AGII, has been rendered environmentally safe by both disease-symptom attenuation and aphid-assisted virus transmission abolishment. The recombinant AGII-Bar virus-encoding cDNA, when inoculated on diverse cucurbits was highly infectious, accumulated to similar levels as AGII, and elicited attenuated AGII-like symptoms. Potted cucurbits inoculated with AGII-Bar became herbicide resistant about a week post-inoculation. Herbicide resistance was sustained in squash over a period of at least 26 days and for at least 60 days in cucumber grown in a net-house under commercial conditions. To test the applicability of AGII-Bar use in a weed-infested field, a controlled experiment including more than 450 plants inoculated with this construct, was performed. Different dosages of glufosinate ammonium were sprayed, 2 weeks after planting, on the foliage of melons, cucumbers, squash, and watermelons. AGII-Bar provided protection to all inoculated plants, of every variety tested, at each dosage applied, including the highest doses that totally eradicated weeds. This study demonstrates that AGII-Bar can be utilized to facilitate weed control in cucurbits and exemplifies the practical potential of attenuated virus-vector use in agriculture. © 2001 Elsevier Science B.V. All rights reserved.
Note:
Related Files :
Agriculture
Cucumis sativus
Cucurbita
DNA
enzymes
herbicides
virus resistance
Zucchini yellow mosaic virus
Show More
Related Content
More details
DOI :
10.1016/S0168-1656(01)00363-7
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24109
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:05
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Scientific Publication
A new approach for weed control in a cucurbit field employing an attenuated potyvirus-vector for herbicide resistance
92
Shiboleth, Y.M., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Arazi, T., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Wang, Y., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
Gal-On, A., Department of Virology, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50-250, Israel
A new approach for weed control in a cucurbit field employing an attenuated potyvirus-vector for herbicide resistance
Expression of bar, a phosphinothricin acetyltransferase, in plant tissues, leads to resistance of these plants to glufosinate ammonium based herbicides. We have created a bar expressing, attenuated zucchini yellow mosaic potyvirus-vector, AGII-Bar, to enable herbicide use in cucurbit fields. The parental vector, ZYMV-AGII, has been rendered environmentally safe by both disease-symptom attenuation and aphid-assisted virus transmission abolishment. The recombinant AGII-Bar virus-encoding cDNA, when inoculated on diverse cucurbits was highly infectious, accumulated to similar levels as AGII, and elicited attenuated AGII-like symptoms. Potted cucurbits inoculated with AGII-Bar became herbicide resistant about a week post-inoculation. Herbicide resistance was sustained in squash over a period of at least 26 days and for at least 60 days in cucumber grown in a net-house under commercial conditions. To test the applicability of AGII-Bar use in a weed-infested field, a controlled experiment including more than 450 plants inoculated with this construct, was performed. Different dosages of glufosinate ammonium were sprayed, 2 weeks after planting, on the foliage of melons, cucumbers, squash, and watermelons. AGII-Bar provided protection to all inoculated plants, of every variety tested, at each dosage applied, including the highest doses that totally eradicated weeds. This study demonstrates that AGII-Bar can be utilized to facilitate weed control in cucurbits and exemplifies the practical potential of attenuated virus-vector use in agriculture. © 2001 Elsevier Science B.V. All rights reserved.
Scientific Publication
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