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Journal of Membrane Biology
Brauer, D.K., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Yermiyahu, U., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Rytwo, G., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Kinraide, T.B., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Several laboratories have determined the surface charge density of membranes utilizing methods based on vesicle-induced quenching of the fluorescence of 9-aminoacridine and its relief by other cations. However, the computational methods by which surface charge density were calculated have not been verified in a model system. In this study, the quenching of 9-aminoacridine fluorescence by liposomes made from varying amounts of digalactosyldiacylglyceride and phosphatidic acid and relief of quenching by salts was examined. Quenching of 9-aminoacridine fluorescence increased with increasing amounts of phosphatidic acid added, independent of the composition of the added liposomes. In certain instances, the computational methods did not yield the surface charge density of the liposomes expected from their composition. However, when the effects of background ionic strength on surface potential were considered, there was a positive correlation between expected and calculated values. Therefore, the data support the contention that changes in the fluorescence of 9-aminoacridine can be used to calculate surface charge density of membranes.
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Characteristics of the quenching of 9-aminoacridine fluorescence by liposomes made from plant lipids
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Brauer, D.K., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Yermiyahu, U., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Rytwo, G., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Kinraide, T.B., Appalachian Farming Systems Research Center, United States Department of Agriculture, 1224 Airport Road, Beaver, WV 25813-0400, United States
Characteristics of the quenching of 9-aminoacridine fluorescence by liposomes made from plant lipids
Several laboratories have determined the surface charge density of membranes utilizing methods based on vesicle-induced quenching of the fluorescence of 9-aminoacridine and its relief by other cations. However, the computational methods by which surface charge density were calculated have not been verified in a model system. In this study, the quenching of 9-aminoacridine fluorescence by liposomes made from varying amounts of digalactosyldiacylglyceride and phosphatidic acid and relief of quenching by salts was examined. Quenching of 9-aminoacridine fluorescence increased with increasing amounts of phosphatidic acid added, independent of the composition of the added liposomes. In certain instances, the computational methods did not yield the surface charge density of the liposomes expected from their composition. However, when the effects of background ionic strength on surface potential were considered, there was a positive correlation between expected and calculated values. Therefore, the data support the contention that changes in the fluorescence of 9-aminoacridine can be used to calculate surface charge density of membranes.
Scientific Publication
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