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Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants
Year:
2008
Authors :
Elad, Yigal
;
.
Katan, Talma
;
.
Korolev, Nadia
;
.
Volume :
122
Co-Authors:
Korolev, N., Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan 50250, Israel
Katan, T., Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan 50250, Israel
Facilitators :
From page:
369
To page:
383
(
Total pages:
15
)
Abstract:
Twenty-one strains of Botrytis cinerea isolated from six plant species on ten sites throughout Israel, as well as a strain from France, were tested for vegetative and mycelial incompatibility, pathogenicity, resistance to the fungicides carbendazim and iprodione, and colony morphology. Selenate-resistant mutants were isolated from the strains as spontaneous, fast-growing sectors arising from restricted colonies on medium amended with sodium selenate with a mean frequency of 0.04 sectors/colony; 81% of the sectors were sulphate non-utilizing (sul) mutants. One hundred and four sul mutants were divided into two complementary groups: resistant (66 mutants) and sensitive to chromate. Based on compatibility reactions between chromate-resistant and chromate-sensitive sul mutants, 12 strains were compatible only with themselves and were each classified as belonging to different vegetative compatibility groups (VCGs). Nine strains were each compatible with one to three other strains, and were assembled into three multi-member VCGs. Mycelial incompatibility between wild-type strains (barrage), in the form of a zone of dark pigmentation or sparse mycelium with or without dark pigmentation of the agar along the line of confrontation, was observed for 70% of the inter-strain pairings. There was no correspondence in compatibility between strains revealed by two approaches: strains in different VCGs did not necessarily produce a barrage. However, self-compatibility was observed both as heterokaryon formation between complementary sul mutants and as an absence of barrages between mycelia of wild-type strains; wild-type strains belonging to the same VCG did not exhibit strong barrages, although weak antagonistic reactions were observed. Strains in two multi-member VCGs showed the same patterns of resistance to carbendazim and iprodione; the third multi-member VCG contained isolates with different patterns of resistance. Four morphological types were revealed among wild-type strains: conidial (five strains), sclerotial (six strains), intermediate (ten strains), and mycelial (one strain). On bean leaves, conidial strains were more aggressive than sclerotial strains. © 2008 KNPV.
Note:
Related Files :
Carbendazim
France
Iprodione
Israel
Morphological type
mutation
Phaseolus vulgaris
sulfate
Vegetation
Show More
Related Content
More details
DOI :
10.1007/s10658-008-9301-6
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24143
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:05
You may also be interested in
Scientific Publication
Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants
122
Korolev, N., Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan 50250, Israel
Elad, Y., Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan 50250, Israel
Katan, T., Department of Plant Pathology and Weed Research, Volcani Center, ARO, Bet Dagan 50250, Israel
Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants
Twenty-one strains of Botrytis cinerea isolated from six plant species on ten sites throughout Israel, as well as a strain from France, were tested for vegetative and mycelial incompatibility, pathogenicity, resistance to the fungicides carbendazim and iprodione, and colony morphology. Selenate-resistant mutants were isolated from the strains as spontaneous, fast-growing sectors arising from restricted colonies on medium amended with sodium selenate with a mean frequency of 0.04 sectors/colony; 81% of the sectors were sulphate non-utilizing (sul) mutants. One hundred and four sul mutants were divided into two complementary groups: resistant (66 mutants) and sensitive to chromate. Based on compatibility reactions between chromate-resistant and chromate-sensitive sul mutants, 12 strains were compatible only with themselves and were each classified as belonging to different vegetative compatibility groups (VCGs). Nine strains were each compatible with one to three other strains, and were assembled into three multi-member VCGs. Mycelial incompatibility between wild-type strains (barrage), in the form of a zone of dark pigmentation or sparse mycelium with or without dark pigmentation of the agar along the line of confrontation, was observed for 70% of the inter-strain pairings. There was no correspondence in compatibility between strains revealed by two approaches: strains in different VCGs did not necessarily produce a barrage. However, self-compatibility was observed both as heterokaryon formation between complementary sul mutants and as an absence of barrages between mycelia of wild-type strains; wild-type strains belonging to the same VCG did not exhibit strong barrages, although weak antagonistic reactions were observed. Strains in two multi-member VCGs showed the same patterns of resistance to carbendazim and iprodione; the third multi-member VCG contained isolates with different patterns of resistance. Four morphological types were revealed among wild-type strains: conidial (five strains), sclerotial (six strains), intermediate (ten strains), and mycelial (one strain). On bean leaves, conidial strains were more aggressive than sclerotial strains. © 2008 KNPV.
Scientific Publication
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