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Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine
Year:
1984
Source of publication :
Plant Cell Reports
Authors :
Izhar, Shamay
;
.
Zelcer, Aaron
;
.
Volume :
3
Co-Authors:
Guri, A., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50 250, Israel
Zelcer, A., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50 250, Israel
Izhar, S., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50 250, Israel
Facilitators :
From page:
219
To page:
221
(
Total pages:
3
)
Abstract:
Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml aphidicolin for 18 h, then cultured in drug-free medium for 8 h and finally exposed to 0.1% colchicine for 8 additional hours exhibited MI of 62.8% and 65.7% respectively, for the two cell lines in study. © 1984 Springer-Verlag.
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More details
DOI :
10.1007/BF00269296
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24201
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:05
Scientific Publication
Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine
3
Guri, A., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50 250, Israel
Zelcer, A., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50 250, Israel
Izhar, S., Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50 250, Israel
Induction of high mitotic index in Petunia suspension cultures by sequential treatment with aphidicolin and colchicine
Significantly higher than normal mitotic index (MI) values were induced in Petunia cell suspensions following treatments with colchicine, aphidicolin, drastic medium replacement, or a sequential application of aphidicolin and colchicine. This last treatment yielded the highest MI values: cells incubated with 30 μg/ml aphidicolin for 18 h, then cultured in drug-free medium for 8 h and finally exposed to 0.1% colchicine for 8 additional hours exhibited MI of 62.8% and 65.7% respectively, for the two cell lines in study. © 1984 Springer-Verlag.
Scientific Publication
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