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Functional analysis of the grapevine virus A genome
Year:
2003
Source of publication :
Virology
Authors :
Gafny, Ron
;
.
Volume :
306
Co-Authors:
Galiakparov, N., Department of Virology, ARO, Volcani Center, Bet-Dagan 50250, Israel, Virus Laboratory, Hebrew University of Jerusalem, Fac. Agric., Food/Environ. Qual. S., Rehovot 76100, Israel
Tanne, E., Department of Virology, ARO, Volcani Center, Bet-Dagan 50250, Israel
Sela, I., Virus Laboratory, Hebrew University of Jerusalem, Fac. Agric., Food/Environ. Qual. S., Rehovot 76100, Israel
Gafny, R., Department of Virology, ARO, Volcani Center, Bet-Dagan 50250, Israel
Facilitators :
From page:
42
To page:
50
(
Total pages:
9
)
Abstract:
Grapevine virus A (GVA) carries five open reading frames (ORFs). Only the coat protein ORF has been experimentally identified as such; the roles of some of the other ORFs have been deduced by sequence homology to known genes (Minafra et al., 1997). The construction of a full-length, infectious clone of GVA has been previously reported (Galiakparov et al., 1999). In an attempt to experimentally define the role of the various genes of GVA, we utilized the infectious clone, inserted mutations in every ORF, and studied the effect on viral replication, gene expression, symptoms and viral movement. Mutations in ORF 1 abolished RNA replication. Mutations in ORF 2 did not affect any of the aforementioned parameters. Mutations in ORFs 3 and 4 restricted viral movement. Mutations in ORF 5 rendered the virus asymptomatic, and partially restricted its movement. © 2003 Elsevier Science (USA). All rights reserved.
Note:
Related Files :
gene expression
infectious clone
mutation
Plant Diseases
RNA viruses
viral genetics
Vitis
Show More
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More details
DOI :
10.1016/S0042-6822(02)00019-3
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24202
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:05
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Scientific Publication
Functional analysis of the grapevine virus A genome
306
Galiakparov, N., Department of Virology, ARO, Volcani Center, Bet-Dagan 50250, Israel, Virus Laboratory, Hebrew University of Jerusalem, Fac. Agric., Food/Environ. Qual. S., Rehovot 76100, Israel
Tanne, E., Department of Virology, ARO, Volcani Center, Bet-Dagan 50250, Israel
Sela, I., Virus Laboratory, Hebrew University of Jerusalem, Fac. Agric., Food/Environ. Qual. S., Rehovot 76100, Israel
Gafny, R., Department of Virology, ARO, Volcani Center, Bet-Dagan 50250, Israel
Functional analysis of the grapevine virus A genome
Grapevine virus A (GVA) carries five open reading frames (ORFs). Only the coat protein ORF has been experimentally identified as such; the roles of some of the other ORFs have been deduced by sequence homology to known genes (Minafra et al., 1997). The construction of a full-length, infectious clone of GVA has been previously reported (Galiakparov et al., 1999). In an attempt to experimentally define the role of the various genes of GVA, we utilized the infectious clone, inserted mutations in every ORF, and studied the effect on viral replication, gene expression, symptoms and viral movement. Mutations in ORF 1 abolished RNA replication. Mutations in ORF 2 did not affect any of the aforementioned parameters. Mutations in ORFs 3 and 4 restricted viral movement. Mutations in ORF 5 rendered the virus asymptomatic, and partially restricted its movement. © 2003 Elsevier Science (USA). All rights reserved.
Scientific Publication
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