נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
IS 1327, a new insertion-like element in the pathogenicity-associated plasmid of Erwinia herbicola pv. gypsophilae
Year:
1996
Authors :
Karniol, Baruch
;
.
Lichter, Amnon
;
.
Manulis-Sasson, Shulamit
;
.
Valinski, Lea
;
.
Volume :
9
Co-Authors:
Lichter, A.
Manulis, S.
Valinsky, L.
Karniol, B.
Barash, I., Department of Botany, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
Facilitators :
From page:
98
To page:
104
(
Total pages:
7
)
Abstract:
The pathogenicity-associated plasmid (pPATH) of Erwinia herbicola pv. gypsophilae was previously shown to be exclusively present in pathogenic strains and to contain a gene cluster encoding phytohormone biosynthesis. Sequence analysis of the DNA region located downstream from the cytokinin biosynthetic gene (etz) revealed homology to insertion sequences (IS) of the IS6 family. Southern blot analysis performed on plasmid DNA of E. herbicola pv. gypsophilae revealed the presence of six copies of this insertion-like element, which was designated as IS1327. Only pathogenic strains contained IS1327 and restriction fragment length polymorphism was observed among gypsophila and beet pathovars of E. herbicola. Nonpathogenic deletion derivative of pPATH contained fewer copies of IS1327, suggesting its presence in the deleted region. One copy of IS1327 (IS1327-R) was located 2.8 kb downstream from the IS element adjacent to the etz (IS1327-L) in a direct repeat.
Note:
Related Files :
Base Sequence
Erwinia
Genetics
molecular genetics
Pantoea agglomerans pv. gypsophilae
Plasmid
Show More
Related Content
More details
DOI :
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24266
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
You may also be interested in
Scientific Publication
IS 1327, a new insertion-like element in the pathogenicity-associated plasmid of Erwinia herbicola pv. gypsophilae
9
Lichter, A.
Manulis, S.
Valinsky, L.
Karniol, B.
Barash, I., Department of Botany, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
IS 1327, a new insertion-like element in the pathogenicity-associated plasmid of Erwinia herbicola pv. gypsophilae
The pathogenicity-associated plasmid (pPATH) of Erwinia herbicola pv. gypsophilae was previously shown to be exclusively present in pathogenic strains and to contain a gene cluster encoding phytohormone biosynthesis. Sequence analysis of the DNA region located downstream from the cytokinin biosynthetic gene (etz) revealed homology to insertion sequences (IS) of the IS6 family. Southern blot analysis performed on plasmid DNA of E. herbicola pv. gypsophilae revealed the presence of six copies of this insertion-like element, which was designated as IS1327. Only pathogenic strains contained IS1327 and restriction fragment length polymorphism was observed among gypsophila and beet pathovars of E. herbicola. Nonpathogenic deletion derivative of pPATH contained fewer copies of IS1327, suggesting its presence in the deleted region. One copy of IS1327 (IS1327-R) was located 2.8 kb downstream from the IS element adjacent to the etz (IS1327-L) in a direct repeat.
Scientific Publication
You may also be interested in