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Isolation, identification, and activity of mycoherbicidal pathogens from juvenile broomrape plants
Year:
2001
Source of publication :
biological control (source)
Authors :
Kleifeld, Yeshaiahu
;
.
Volume :
21
Co-Authors:
Amsellem, Z., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, IL-76100, Israel
Kleifeld, Y., Department of Weed Science, Newe Yaar Research Center, Ramat-Ishay, Israel
Kerenyi, Z., Plant Sciences, Agricultural Biotechnology Center (ABC), Gödöllö, Hungary
Hornok, L., Plant Sciences, Agricultural Biotechnology Center (ABC), Gödöllö, Hungary
Goldwasser, Y., Department of Weed Science, Newe Yaar Research Center, Ramat-Ishay, Israel
Gressel, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, IL-76100, Israel
Facilitators :
From page:
274
To page:
284
(
Total pages:
11
)
Abstract:
Although there are reports of isolation of mycoherbicidal pathogens attacking the widespread broomrapes (Orobanche spp.) that parasitize legumes and vegetables, none is in use or available. This is despite there being no good method of controlling broomrapes in most crops other than by preplant fumigation with methyl bromide. Two highly parasitic fungi, Fusarium arthrosporioides strain E4a (CNCM 1-164) and F. oxysporum strain Eld (CNCM 1-1622), were isolated from nearly 100 organisms found on diseased, juvenile, emerging Orobanche flower stalks. A near-axenic polyethylene envelope system for culturing broomrape on tomato roots was used to ascertain pathogenicity of these strains. Both organisms fulfilled Koch's postulates for being primary pathogens. Their DNAs were analyzed and fingerprinted by restriction fragment length polymorphism and random amplified polymorphic DNA, showing that they are indeed different from each other and from many other Fusarium spp. and other formae speciales of F. oxysporum including a strain that attacks O. cumana on sunflowers. Both strains infect O. aegyptiaca, O. cernua, and O. ramosa, but not O. cumana. They did not infect any of the vegetable and legume crops tested and thus seem specific to Orobanche. Tomato plant roots dipped into a fungal spore and mycelial suspension and planted in broomrape-infested soil were protected for 6 weeks, as were tomato transplants in pot experiments. About 90% control was also achieved by posttransplant soil drench with fungal suspensions in pot experiments. These pathogens may be effective as seed, transplant, or soil-drench treatments of high-value vegetable and other crops. © 2001 Academic Press.
Note:
Related Files :
fungi
Fusarium arthrosporioides
methyl bromide
Microbial herbicides
Orobanche ramosa
polyethylene
Show More
Related Content
More details
DOI :
10.1006/bcon.2001.0934
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24337
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
Scientific Publication
Isolation, identification, and activity of mycoherbicidal pathogens from juvenile broomrape plants
21
Amsellem, Z., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, IL-76100, Israel
Kleifeld, Y., Department of Weed Science, Newe Yaar Research Center, Ramat-Ishay, Israel
Kerenyi, Z., Plant Sciences, Agricultural Biotechnology Center (ABC), Gödöllö, Hungary
Hornok, L., Plant Sciences, Agricultural Biotechnology Center (ABC), Gödöllö, Hungary
Goldwasser, Y., Department of Weed Science, Newe Yaar Research Center, Ramat-Ishay, Israel
Gressel, J., Department of Plant Sciences, Weizmann Institute of Science, Rehovot, IL-76100, Israel
Isolation, identification, and activity of mycoherbicidal pathogens from juvenile broomrape plants
Although there are reports of isolation of mycoherbicidal pathogens attacking the widespread broomrapes (Orobanche spp.) that parasitize legumes and vegetables, none is in use or available. This is despite there being no good method of controlling broomrapes in most crops other than by preplant fumigation with methyl bromide. Two highly parasitic fungi, Fusarium arthrosporioides strain E4a (CNCM 1-164) and F. oxysporum strain Eld (CNCM 1-1622), were isolated from nearly 100 organisms found on diseased, juvenile, emerging Orobanche flower stalks. A near-axenic polyethylene envelope system for culturing broomrape on tomato roots was used to ascertain pathogenicity of these strains. Both organisms fulfilled Koch's postulates for being primary pathogens. Their DNAs were analyzed and fingerprinted by restriction fragment length polymorphism and random amplified polymorphic DNA, showing that they are indeed different from each other and from many other Fusarium spp. and other formae speciales of F. oxysporum including a strain that attacks O. cumana on sunflowers. Both strains infect O. aegyptiaca, O. cernua, and O. ramosa, but not O. cumana. They did not infect any of the vegetable and legume crops tested and thus seem specific to Orobanche. Tomato plant roots dipped into a fungal spore and mycelial suspension and planted in broomrape-infested soil were protected for 6 weeks, as were tomato transplants in pot experiments. About 90% control was also achieved by posttransplant soil drench with fungal suspensions in pot experiments. These pathogens may be effective as seed, transplant, or soil-drench treatments of high-value vegetable and other crops. © 2001 Academic Press.
Scientific Publication
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