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Molecular evolution of Turnip mosaic virus: Evidence of host adaptation, genetic recombination and geographical spread
Year:
2002
Source of publication :
Journal of General Virology
Authors :
Gera, Abdullah
;
.
Volume :
83
Co-Authors:
Ohshima, K., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Yamaguchi, Y., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Hirota, R., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Hamamoto, T., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Tomimura, K., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Tan, Z., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Sano, T., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Azuhata, F., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Walsh, J.A., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Fletcher, J., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Chen, J., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Gera, A., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Gibbs, A., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Facilitators :
From page:
1511
To page:
1521
(
Total pages:
11
)
Abstract:
Turnip mosaic virus (TuMV), a species of the genus Potyvirus, occurs worldwide. Seventy-six isolates of TuMV were collected from around the world, mostly from Brassica and Raphanus crops, but also from several non-brassica species. Host tests grouped the isolates into one or other of two pathotypes; Brassica (B) and Brassica-Raphanus (BR). The nucleotide sequences of the first protein (P1) and coat protein (CP) genes of the isolates were determined. One-tenth of the isolates were found to have anomalous and variable phylogenetic relationships as a result of recombination. The 5′-terminal 300 nt of the P1 gene of many isolates was also variable and phylogenetically anomalous, whereas the 380 nt 3′ terminus of the CP gene was mostly conserved. Trees calculated from the remaining informative parts of the two genes of the non-recombinant sequences by neighbour-joining, maximum-likelihood and maximum-parsimony methods were closely similar, and so these parts of the sequences were concatenated and trees calculated from the resulting 1150 nt. The isolates fell into four consistent groups; only the relationships of these groups with one another and with the outgroup differed. The 'basal-B' cluster of eight B-pathotype isolates was most variable, was not monophyletic, and came from both brassicas and non-brassicas from southwest and central Eurasia. Closest to it, and forming a monophyletic subgroup of it in most trees, and similarly variable, was the 'basal-BR' group of eight BR pathotype Eurasian isolates. The third and least variable group, the 'Asian-BR' group, was of 22 BR-pathotype isolates, all from brassicas, mostly Raphanus, and all from east Asia mostly Japan. The fourth group of 36 isolates, the 'world-B' group, was from all continents, most were isolated from brassicas and most were of the B-pathotype. The simplest of several possible interpretations of the trees is that TuMV originated, like its brassica hosts, in Europe and spread to the other parts of the world, and that the BR pathotype has recently evolved in east Asia.
Note:
Related Files :
Brassicaceae
Brassica rapa subsp. rapa
genetic recombination
Molecular Evolution
Raphanus sativus
Show More
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24342
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
Scientific Publication
Molecular evolution of Turnip mosaic virus: Evidence of host adaptation, genetic recombination and geographical spread
83
Ohshima, K., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Yamaguchi, Y., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Hirota, R., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Hamamoto, T., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Tomimura, K., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Tan, Z., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Sano, T., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Azuhata, F., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Walsh, J.A., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Fletcher, J., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Chen, J., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Gera, A., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Gibbs, A., Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan
Molecular evolution of Turnip mosaic virus: Evidence of host adaptation, genetic recombination and geographical spread
Turnip mosaic virus (TuMV), a species of the genus Potyvirus, occurs worldwide. Seventy-six isolates of TuMV were collected from around the world, mostly from Brassica and Raphanus crops, but also from several non-brassica species. Host tests grouped the isolates into one or other of two pathotypes; Brassica (B) and Brassica-Raphanus (BR). The nucleotide sequences of the first protein (P1) and coat protein (CP) genes of the isolates were determined. One-tenth of the isolates were found to have anomalous and variable phylogenetic relationships as a result of recombination. The 5′-terminal 300 nt of the P1 gene of many isolates was also variable and phylogenetically anomalous, whereas the 380 nt 3′ terminus of the CP gene was mostly conserved. Trees calculated from the remaining informative parts of the two genes of the non-recombinant sequences by neighbour-joining, maximum-likelihood and maximum-parsimony methods were closely similar, and so these parts of the sequences were concatenated and trees calculated from the resulting 1150 nt. The isolates fell into four consistent groups; only the relationships of these groups with one another and with the outgroup differed. The 'basal-B' cluster of eight B-pathotype isolates was most variable, was not monophyletic, and came from both brassicas and non-brassicas from southwest and central Eurasia. Closest to it, and forming a monophyletic subgroup of it in most trees, and similarly variable, was the 'basal-BR' group of eight BR pathotype Eurasian isolates. The third and least variable group, the 'Asian-BR' group, was of 22 BR-pathotype isolates, all from brassicas, mostly Raphanus, and all from east Asia mostly Japan. The fourth group of 36 isolates, the 'world-B' group, was from all continents, most were isolated from brassicas and most were of the B-pathotype. The simplest of several possible interpretations of the trees is that TuMV originated, like its brassica hosts, in Europe and spread to the other parts of the world, and that the BR pathotype has recently evolved in east Asia.
Scientific Publication
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