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Anti-leptin receptor antibodies strengthen leptin biofunction in growing chickens
Year:
2017
Authors :
Yosefi, Sara
;
.
Volume :
259
Co-Authors:
Lei, M.M., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Wei, C.K., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Chen, Z., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Yosefi, S., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Zhu, H.X., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Shi, Z.D., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Facilitators :
From page:
223
To page:
230
(
Total pages:
8
)
Abstract:
Antibodies against the extracellular domains of the chicken leptin receptor were used to study the biological function of leptin in growing chickens. Both polyclonal and monoclonal anti-LEPR antibodies were administered intramuscularly to 30-d-old Chinese indigenous Gushi pullets. Both antibody preparations increased feed intake for 6 h after injection and reduced plasma concentrations of glucose, triglycerides, and both high- and low-density lipoproteins. The antibody treatments also upregulated agouti-related peptide and neuropeptide Y in the hypothalamus and downregulated proopiomelanocortin, melanocortin 4 receptor, and leptin receptor. The treatments also upregulated leptin receptor, acetyl CoA carboxylase beta, and acyl-CoA oxidase in the liver, abdominal fat, and breast muscle and downregulated sterol regulatory element-binding protein-1 and fatty acid synthase. Furthermore, even though the anti-leptin receptor antibodies failed to affect leptin receptor signaling transduction when administered alone, they did augment the induction of leptin receptor signaling transduction by leptin. These results demonstrate that antibodies against the extracellular domains of leptin-specific receptor enhance, but do not mimic, the ability of leptin to activate receptors. Furthermore, the enhanced leptin bioactivity observed after the intramuscular injection of anti-LEPR antibodies confirmed the occurrence of de novo leptin in the peripheral tissues and blood of treated chickens. © 2017 Elsevier Inc.
Note:
Related Files :
Anti-leptin receptor antibodies
Growing chicken
Leptin bioactivity
Leptin receptor signal transduction
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Related Content
More details
DOI :
10.1016/j.ygcen.2017.12.005
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24368
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
Scientific Publication
Anti-leptin receptor antibodies strengthen leptin biofunction in growing chickens
259
Lei, M.M., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Wei, C.K., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Chen, Z., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Yosefi, S., Institute of Animal Science, Agricultural Research Organization, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Zhu, H.X., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Shi, Z.D., Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality, MOA, Nanjing 210014, China, Laboratory of Animal Breeding and Reproduction, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
Anti-leptin receptor antibodies strengthen leptin biofunction in growing chickens
Antibodies against the extracellular domains of the chicken leptin receptor were used to study the biological function of leptin in growing chickens. Both polyclonal and monoclonal anti-LEPR antibodies were administered intramuscularly to 30-d-old Chinese indigenous Gushi pullets. Both antibody preparations increased feed intake for 6 h after injection and reduced plasma concentrations of glucose, triglycerides, and both high- and low-density lipoproteins. The antibody treatments also upregulated agouti-related peptide and neuropeptide Y in the hypothalamus and downregulated proopiomelanocortin, melanocortin 4 receptor, and leptin receptor. The treatments also upregulated leptin receptor, acetyl CoA carboxylase beta, and acyl-CoA oxidase in the liver, abdominal fat, and breast muscle and downregulated sterol regulatory element-binding protein-1 and fatty acid synthase. Furthermore, even though the anti-leptin receptor antibodies failed to affect leptin receptor signaling transduction when administered alone, they did augment the induction of leptin receptor signaling transduction by leptin. These results demonstrate that antibodies against the extracellular domains of leptin-specific receptor enhance, but do not mimic, the ability of leptin to activate receptors. Furthermore, the enhanced leptin bioactivity observed after the intramuscular injection of anti-LEPR antibodies confirmed the occurrence of de novo leptin in the peripheral tissues and blood of treated chickens. © 2017 Elsevier Inc.
Scientific Publication
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