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Overexpression of a peach defensin gene can enhance the activity of postharvest biocontrol agents
Year:
2005
Source of publication :
Acta Horticulturae
Authors :
Droby, Samir
;
.
Volume :
682
Co-Authors:
Wisniewski, M., USDA, ARS, Kearneysville, WV 25430, United States
Bassett, C., USDA, ARS, Kearneysville, WV 25430, United States
Artlip, T., USDA, ARS, Kearneysville, WV 25430, United States
Janisiewicz, W., USDA, ARS, Kearneysville, WV 25430, United States
Norelli, J., USDA, ARS, Kearneysville, WV 25430, United States
Goldway, M., MIGAL, Galilee Technological Center, Rosh Pina, Israel
Droby, S., ARO, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
1999
To page:
2006
(
Total pages:
8
)
Abstract:
A portion of our research involves developing methods to enhance the activity of yeast biocontrol agents. In the present study, we describe the cloning and characterization of defensin genes from bark tissues of peach and apple. The open reading frame of both genes code for proteins related to the defensins, a family of highly antimicrobial proteins. Sequence comparison of the encoded proteins revealed significant homology between the peach and apple defensins and defensins from other plant species. RNA gel blot analysis and semi-quantitative-PCR indicated that the genes are seasonally-expressed in bark tissues of one-year-old shoots, and the peach defensin is also expressed in early fruit development. A recombinant version of the peach defensin gene, rDFNl was expressed in the yeast, Pichia pastoris. It was found that rDFNl inhibited germination of the fungal pathogens Pénicillium expansum and Botrytis cinerea. The potential of using defensin genes to enhance biocontrol yeasts is discussed.
Note:
Related Files :
Malus domestica
Pathogenesis-related-proteins
Penicillium expansum
Postharvest disease
Prunus persica
yeasts
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Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
Conference paper
;
.
Language:
English
Editors' remarks:
ID:
24372
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:06
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Scientific Publication
Overexpression of a peach defensin gene can enhance the activity of postharvest biocontrol agents
682
Wisniewski, M., USDA, ARS, Kearneysville, WV 25430, United States
Bassett, C., USDA, ARS, Kearneysville, WV 25430, United States
Artlip, T., USDA, ARS, Kearneysville, WV 25430, United States
Janisiewicz, W., USDA, ARS, Kearneysville, WV 25430, United States
Norelli, J., USDA, ARS, Kearneysville, WV 25430, United States
Goldway, M., MIGAL, Galilee Technological Center, Rosh Pina, Israel
Droby, S., ARO, Volcani Center, Bet Dagan, Israel
Overexpression of a peach defensin gene can enhance the activity of postharvest biocontrol agents
A portion of our research involves developing methods to enhance the activity of yeast biocontrol agents. In the present study, we describe the cloning and characterization of defensin genes from bark tissues of peach and apple. The open reading frame of both genes code for proteins related to the defensins, a family of highly antimicrobial proteins. Sequence comparison of the encoded proteins revealed significant homology between the peach and apple defensins and defensins from other plant species. RNA gel blot analysis and semi-quantitative-PCR indicated that the genes are seasonally-expressed in bark tissues of one-year-old shoots, and the peach defensin is also expressed in early fruit development. A recombinant version of the peach defensin gene, rDFNl was expressed in the yeast, Pichia pastoris. It was found that rDFNl inhibited germination of the fungal pathogens Pénicillium expansum and Botrytis cinerea. The potential of using defensin genes to enhance biocontrol yeasts is discussed.
Scientific Publication
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