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Protective effects of iodixanol during bovine sperm cryopreservation
Year:
2009
Source of publication :
Theriogenology
Authors :
Gacitua, Haim
;
.
Volume :
71
Co-Authors:
Saragusty, J., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel, Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, P.O. Box 12, 76100 Rehovot, Israel
Gacitua, H., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Rozenboim, I., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, P.O. Box 12, 76100 Rehovot, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Facilitators :
From page:
1425
To page:
1432
(
Total pages:
8
)
Abstract:
The aim of cryopreservation is to maintain cellular integrity, thereby enabling resumption of proper biological functioning after thawing. Here we propose OptiPrep™ (60% iodixanol in water) as a protectant during sperm cryopreservation using pooled bull semen as the model. We evaluated OptiPrep concentration effect and its relation to cryopreservation by comparing frozen-thawed and chilled samples. Semen, extended in Andromed® with 0 (control), 1.25%, 2.5%, and 5% OptiPrep™, was compared after either chilling or freezing in large volume by directional freezing. Sample evaluation included sperm motility upon thawing and after 3 h incubation at 37 °C for frozen-thawed samples and after 3 h and 6 h of chilling for chilled samples; viability, acrosomal integrity, and hypoosmotic swelling were also tested for frozen-thawed and chilled samples. Chilled samples with 5% OptiPrep™ showed inferior viability (P = 0.047) and 3 h motility (P = 0.017) relative to that for chilled samples with 2.5% OptiPrep and inferior viability (P = 0.042), acrosomal integrity (P = 0.045), and 0 h motility (P = 0.024) relative to that for chilled samples with 1.25% OptiPrep. The 1.25%, 2.5%, and control samples did not differ. In frozen-thawed samples, 2.5% OptiPrep was superior to all other concentrations for 3 h motility (control, P = 0.007; 5% OptiPrep, P = 0.005; 1.25% OptiPrep, P = 0.004) and to 1.25% OptiPrep for acrosomal integrity (P = 0.001). In a search for a protection mechanism, we measured glass transition temperature (Tg) of Andromed® and of Andromed® with 1.25%, 2.5%, and 5% OptiPrep™. Andromed® (-58.78 °C) and 1.25% OptiPrep™ (-58.75 °C) groups had lower mean Tg than that of the 2.5% (-57.67 °C) and the 5% (-57.10 °C) groups. Directional cryomicroscopy revealed that the presence of iodixanol alters ice crystal formation into an intricate net of dendrites. Thus, iodixanol appears to possess cryoprotective properties by helping spermatozoa maintain motility and membrane integrity, possibly through altering ice crystals formation into a more hospitable environment and increasing the glass transition temperature. © 2009 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animal
Animals
cattle
chemistry
Cushioned centrifugation
Male
Semen Preservation
Show More
Related Content
More details
DOI :
10.1016/j.theriogenology.2009.01.019
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24384
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:07
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Scientific Publication
Protective effects of iodixanol during bovine sperm cryopreservation
71
Saragusty, J., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel, Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, P.O. Box 12, 76100 Rehovot, Israel
Gacitua, H., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Rozenboim, I., Department of Animal Sciences, Faculty of Agricultural, Food and Environmental Quality Sciences, P.O. Box 12, 76100 Rehovot, Israel
Arav, A., Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Protective effects of iodixanol during bovine sperm cryopreservation
The aim of cryopreservation is to maintain cellular integrity, thereby enabling resumption of proper biological functioning after thawing. Here we propose OptiPrep™ (60% iodixanol in water) as a protectant during sperm cryopreservation using pooled bull semen as the model. We evaluated OptiPrep concentration effect and its relation to cryopreservation by comparing frozen-thawed and chilled samples. Semen, extended in Andromed® with 0 (control), 1.25%, 2.5%, and 5% OptiPrep™, was compared after either chilling or freezing in large volume by directional freezing. Sample evaluation included sperm motility upon thawing and after 3 h incubation at 37 °C for frozen-thawed samples and after 3 h and 6 h of chilling for chilled samples; viability, acrosomal integrity, and hypoosmotic swelling were also tested for frozen-thawed and chilled samples. Chilled samples with 5% OptiPrep™ showed inferior viability (P = 0.047) and 3 h motility (P = 0.017) relative to that for chilled samples with 2.5% OptiPrep and inferior viability (P = 0.042), acrosomal integrity (P = 0.045), and 0 h motility (P = 0.024) relative to that for chilled samples with 1.25% OptiPrep. The 1.25%, 2.5%, and control samples did not differ. In frozen-thawed samples, 2.5% OptiPrep was superior to all other concentrations for 3 h motility (control, P = 0.007; 5% OptiPrep, P = 0.005; 1.25% OptiPrep, P = 0.004) and to 1.25% OptiPrep for acrosomal integrity (P = 0.001). In a search for a protection mechanism, we measured glass transition temperature (Tg) of Andromed® and of Andromed® with 1.25%, 2.5%, and 5% OptiPrep™. Andromed® (-58.78 °C) and 1.25% OptiPrep™ (-58.75 °C) groups had lower mean Tg than that of the 2.5% (-57.67 °C) and the 5% (-57.10 °C) groups. Directional cryomicroscopy revealed that the presence of iodixanol alters ice crystal formation into an intricate net of dendrites. Thus, iodixanol appears to possess cryoprotective properties by helping spermatozoa maintain motility and membrane integrity, possibly through altering ice crystals formation into a more hospitable environment and increasing the glass transition temperature. © 2009 Elsevier Inc. All rights reserved.
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