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Transgenic expression of tomato bushy stunt virus silencing suppressor P19 via the pOp/LhG4 transactivation system induces viral-like symptoms in tomato
Year:
2010
Source of publication :
Virus Genes
Authors :
Arazi, Tzahi
;
.
Hendelman, Anat
;
.
Stav, Ran
;
.
Volume :
40
Co-Authors:
Stav, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Hendelman, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Buxdorf, K., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Arazi, T., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
119
To page:
129
(
Total pages:
11
)
Abstract:
During natural infection, the Tomato bushy stunt virus (TBSV) silencing suppressor protein P19 is expressed at high levels, which are required for optimum viral pathogenicity and silencing suppression. To date, expression of P19 in transgenic host plants has failed to achieve comparable expression levels and thus has provided only limited information on its in planta effects. To obtain high P19 expression and study its effects on host plant development in the absence of virus infection, we generated HA-tagged P19 (P19HA)-transgenic tomato reporter plants using the pOp/LhG4 transactivation system, which separates transformation from transgene expression. Upon reporter plant activation with a strong constitutive promoter, the transactivated F1 plants expressed high levels of a functional P19HA protein and displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms described previously for TBSV-infected tomato. Moreover, phenotype severity correlated with P19HA expression level, amount of bound miRNA/miRNA duplexes, and accumulation of miRNA target transcripts. Together our results demonstrate that the tomato miRNA pathway is markedly compromised by P19, in particular when this protein is relatively abundant, as occurs during natural infection. We suggest that such interference with endogenous silencing may be responsible for at least some of the symptoms characteristic of TBSV-infected tomato. © 2009 Springer Science+Business Media, LLC.
Note:
Related Files :
Development
gene expression
gene silencing
microRNA
phenotype
Transgenes
virus infection
Show More
Related Content
More details
DOI :
10.1007/s11262-009-0415-5
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24385
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:07
Scientific Publication
Transgenic expression of tomato bushy stunt virus silencing suppressor P19 via the pOp/LhG4 transactivation system induces viral-like symptoms in tomato
40
Stav, R., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Hendelman, A., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Buxdorf, K., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Arazi, T., Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Transgenic expression of tomato bushy stunt virus silencing suppressor P19 via the pOp/LhG4 transactivation system induces viral-like symptoms in tomato
During natural infection, the Tomato bushy stunt virus (TBSV) silencing suppressor protein P19 is expressed at high levels, which are required for optimum viral pathogenicity and silencing suppression. To date, expression of P19 in transgenic host plants has failed to achieve comparable expression levels and thus has provided only limited information on its in planta effects. To obtain high P19 expression and study its effects on host plant development in the absence of virus infection, we generated HA-tagged P19 (P19HA)-transgenic tomato reporter plants using the pOp/LhG4 transactivation system, which separates transformation from transgene expression. Upon reporter plant activation with a strong constitutive promoter, the transactivated F1 plants expressed high levels of a functional P19HA protein and displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms described previously for TBSV-infected tomato. Moreover, phenotype severity correlated with P19HA expression level, amount of bound miRNA/miRNA duplexes, and accumulation of miRNA target transcripts. Together our results demonstrate that the tomato miRNA pathway is markedly compromised by P19, in particular when this protein is relatively abundant, as occurs during natural infection. We suggest that such interference with endogenous silencing may be responsible for at least some of the symptoms characteristic of TBSV-infected tomato. © 2009 Springer Science+Business Media, LLC.
Scientific Publication
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