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Physiological mechanisms of pheromonostatic responses: Effects of adrenergic agonists and antagonists on moth (helicoverpa armigera) pheromone biosynthesis
Year:
1997
Source of publication :
Journal of Insect Physiology
Authors :
Gileadi, Carina
;
.
Rafaeli, Ada
;
.
Volume :
43
Co-Authors:
Rafaeli, A., Department of Stored Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Gileadi, C., Department of Stored Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Yongliang, F., Department of Stored Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel, Shanghai Institute of Entomology, Academia Sinica, China
Cao, M., Shanghai Institute of Entomology, Academia Sinica, China
Facilitators :
From page:
261
To page:
269
(
Total pages:
9
)
Abstract:
The adrenergic agonists octopamine, tyramine and clonidine inhibited the normal pheromonotropic action due to PBAN (pheromone biosynthesis activating neuropeptide) in incubations of intersegmental tissues that are situated between the 8th and 9th abdominal segments of the moth ovipositor tip. This inhibition was reversed in the presence of the adrenergic antagonists phentolamine, yohimbine and chlorpromazine. Incubations of 8th segments alone, which do not produce pheromone, resulted in elevated levels of intracellular cAMP in the presence of octopamine. The physiological significance of this phenomenon is unclear. However, clonidine (an α2 selective agonist) did not duplicate octopamine stimulation of intracellular cAMP in 8th segment cultures. In intersegmental membrane cultures clonidine successfully duplicated the octopamine inhibition of both pheromone and intracellular cAMP production. The physiological significance of octopaminergic receptors mediating the inhibitory response of intersegments was investigated by experiments in vivo. When PBAN was injected into photophase females the normal pheromonotropic activity due to the injected PBAN dropped after 2h. In the presence of clonidine, normal peak stimulatory levels were never attained and a faster decline was observed. Clonidine also inhibited the pheromonotropic response of 24h-decapitated females to PBAN. Adrenergic antagonists successfully reversed the inhibitory effect of clonidine in decapitated females, but did not reverse the effect of clonidine in photophase females. In addition, when clonidine was injected into female moths during the scotophase normal peak pheromone titers were reduced although no effect on calling behavior was observed.
Note:
Related Files :
Adrenergic antagonists
Biogenic amines
clonidine
Helicoverpa armigera
Lepidoptera
PBAN
Pheromone production
Show More
Related Content
More details
DOI :
10.1016/S0022-1910(96)00088-1
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24404
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:07
You may also be interested in
Scientific Publication
Physiological mechanisms of pheromonostatic responses: Effects of adrenergic agonists and antagonists on moth (helicoverpa armigera) pheromone biosynthesis
43
Rafaeli, A., Department of Stored Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Gileadi, C., Department of Stored Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel
Yongliang, F., Department of Stored Products, Volcani Center, PO Box 6, Bet Dagan 50250, Israel, Shanghai Institute of Entomology, Academia Sinica, China
Cao, M., Shanghai Institute of Entomology, Academia Sinica, China
Physiological mechanisms of pheromonostatic responses: Effects of adrenergic agonists and antagonists on moth (helicoverpa armigera) pheromone biosynthesis
The adrenergic agonists octopamine, tyramine and clonidine inhibited the normal pheromonotropic action due to PBAN (pheromone biosynthesis activating neuropeptide) in incubations of intersegmental tissues that are situated between the 8th and 9th abdominal segments of the moth ovipositor tip. This inhibition was reversed in the presence of the adrenergic antagonists phentolamine, yohimbine and chlorpromazine. Incubations of 8th segments alone, which do not produce pheromone, resulted in elevated levels of intracellular cAMP in the presence of octopamine. The physiological significance of this phenomenon is unclear. However, clonidine (an α2 selective agonist) did not duplicate octopamine stimulation of intracellular cAMP in 8th segment cultures. In intersegmental membrane cultures clonidine successfully duplicated the octopamine inhibition of both pheromone and intracellular cAMP production. The physiological significance of octopaminergic receptors mediating the inhibitory response of intersegments was investigated by experiments in vivo. When PBAN was injected into photophase females the normal pheromonotropic activity due to the injected PBAN dropped after 2h. In the presence of clonidine, normal peak stimulatory levels were never attained and a faster decline was observed. Clonidine also inhibited the pheromonotropic response of 24h-decapitated females to PBAN. Adrenergic antagonists successfully reversed the inhibitory effect of clonidine in decapitated females, but did not reverse the effect of clonidine in photophase females. In addition, when clonidine was injected into female moths during the scotophase normal peak pheromone titers were reduced although no effect on calling behavior was observed.
Scientific Publication
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