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The effect of ribose 5-phosphate and 5-phosphoribosyl-1-pyrophosphate availability on de novo synthesis of purine nucleotides in rat liver slices
Year:
1976
Authors :
Lipstein, Bianka
;
.
Volume :
432
Co-Authors:
Boer, P., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Lipstein, B., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
De Vries, A., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Sperling, O., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Facilitators :
From page:
10
To page:
17
(
Total pages:
8
)
Abstract:
The effect of increasing cellular ribose 5-phosphate (ribose-5-P) availability by methylene blue-induced acceleration of the oxidative pentose phosphate pathway, on the rate of 5-phosphoribosyl-1-pyrophosphate (P-ribose-PP) generation, was studied in slices of rat liver at varying Pi concentration. It was found that at Pi concentration prevailing in the tissue at extracellular physiological Pi concentration, ribose-5-P availability is saturating for P-ribose-PP generation, as gauged by the rate of adenine incorporation into tissue nucleotides. The effect of altering P-ribose-PP availability on the rate of de novo purine production gauged by the rate of formate incorporation into purines, was also studied. It was found that the physiological P-ribose-PP concentration in rat liver tissue is limiting for purine synthesis de novo. Depletion of cellular P-ribose-PP, achieved by increase of P-ribose-PP consumption, decelerated purine synthesis, while increase of P-ribose-PP availability, achieved by activation of P-ribose-PP synthetase occurring at elevated Pi concentration, resulted in acceleration of purine synthesis. © 1976.
Note:
Related Files :
adenine c 14
Animal
azaserine
Female
hypoxanthine
In vitro
liver
Purine Nucleotides
ribose 5 phosphate
Show More
Related Content
More details
DOI :
10.1016/0005-2787(76)90036-8
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24445
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:07
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Scientific Publication
The effect of ribose 5-phosphate and 5-phosphoribosyl-1-pyrophosphate availability on de novo synthesis of purine nucleotides in rat liver slices
432
Boer, P., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Lipstein, B., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
De Vries, A., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
Sperling, O., Metabolic Unit, Rogoff-Wellcome Medical Research Institute, Beilinson Medical Center, Petah Tikva, Israel
The effect of ribose 5-phosphate and 5-phosphoribosyl-1-pyrophosphate availability on de novo synthesis of purine nucleotides in rat liver slices
The effect of increasing cellular ribose 5-phosphate (ribose-5-P) availability by methylene blue-induced acceleration of the oxidative pentose phosphate pathway, on the rate of 5-phosphoribosyl-1-pyrophosphate (P-ribose-PP) generation, was studied in slices of rat liver at varying Pi concentration. It was found that at Pi concentration prevailing in the tissue at extracellular physiological Pi concentration, ribose-5-P availability is saturating for P-ribose-PP generation, as gauged by the rate of adenine incorporation into tissue nucleotides. The effect of altering P-ribose-PP availability on the rate of de novo purine production gauged by the rate of formate incorporation into purines, was also studied. It was found that the physiological P-ribose-PP concentration in rat liver tissue is limiting for purine synthesis de novo. Depletion of cellular P-ribose-PP, achieved by increase of P-ribose-PP consumption, decelerated purine synthesis, while increase of P-ribose-PP availability, achieved by activation of P-ribose-PP synthetase occurring at elevated Pi concentration, resulted in acceleration of purine synthesis. © 1976.
Scientific Publication
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