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Pheromonotropic and melanotropic PK/PBAN receptors: Differential ligand-receptor interactions
Year:
2015
Source of publication :
peptides ( source)
Authors :
Altstein, Miriam
;
.
Hariton, Aliza
;
.
Volume :
63
Co-Authors:

Shalev, A.H., Department of Entomology, Volcani Center, PO Box 6, Bet Dagan, Israel
Altstein, M., Department of Entomology, Volcani Center, PO Box 6, Bet Dagan, Israel
 

Facilitators :
From page:
81
To page:
89
(
Total pages:
9
)
Abstract:
The aim of the present study was to further characterize the PK/PBAN receptors and their interaction with various PK/PBAN peptides in order to get a better understanding of their ubiquitous and multifunctional nature. Two cloned receptors stably expressed in Spodoptera frugiperda (Sf9) cells were used in this study: a Heliothis peltigera pheromone gland receptor (Hep-PK/PBAN-R) (which stimulates sex pheromone biosynthesis) and Spodoptera littoralis larval receptor (Spl-PK/PBAN-R) (which mediates cuticular melanization in moth larvae) and their ability to respond to several native PK/PBAN peptides: β-subesophageal neuropeptide (β-SGNP), myotropin (MT) and Leucophaea maderae pyrokinin (LPK), as well as linear and cyclic analogs was tested by monitoring their ability to stimulate Ca2+ release. The receptors exhibited a differential response to β-SGNP, which activated the Hep-PK/PBAN-R but not the Spl-PK/PBAN-R - a response opposite to that previously demonstrated with diapause hormone (DH). MT was somewhat more active on Spl-PK/PBAN-R than on Hep-PK/PBAN-R. LPK elicited similar positive responses in both receptors (like that with PBAN). A differential response toward both receptors was also noticed with the PBAN-derived backbone cyclic (BBC) conformationally constrained peptide BBC-5. The peptides BBC-7 and BBC-8 activated both receptors. The results correlate between two PK/PBAN mediated function (cuticular melanization and sex pheromone biosynthesis) and the peptides that activate them and thus advance our understanding of the mode of action of the PK/PBAN family, and might help in exploring novel high-affinity receptor-specific antagonists that could serve as a basis for development of new families of insect-control agents. © 2014 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animal
Animals
Lepidoptera
Moths
neuropeptide
PBAN Heliothis peltigera
Peltigera
pheromones
unclassified drug
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Related Content
More details
DOI :
10.1016/j.peptides.2014.10.014
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24500
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:08
You may also be interested in
Scientific Publication
Pheromonotropic and melanotropic PK/PBAN receptors: Differential ligand-receptor interactions
63

Shalev, A.H., Department of Entomology, Volcani Center, PO Box 6, Bet Dagan, Israel
Altstein, M., Department of Entomology, Volcani Center, PO Box 6, Bet Dagan, Israel
 

Pheromonotropic and melanotropic PK/PBAN receptors: Differential ligand-receptor interactions
The aim of the present study was to further characterize the PK/PBAN receptors and their interaction with various PK/PBAN peptides in order to get a better understanding of their ubiquitous and multifunctional nature. Two cloned receptors stably expressed in Spodoptera frugiperda (Sf9) cells were used in this study: a Heliothis peltigera pheromone gland receptor (Hep-PK/PBAN-R) (which stimulates sex pheromone biosynthesis) and Spodoptera littoralis larval receptor (Spl-PK/PBAN-R) (which mediates cuticular melanization in moth larvae) and their ability to respond to several native PK/PBAN peptides: β-subesophageal neuropeptide (β-SGNP), myotropin (MT) and Leucophaea maderae pyrokinin (LPK), as well as linear and cyclic analogs was tested by monitoring their ability to stimulate Ca2+ release. The receptors exhibited a differential response to β-SGNP, which activated the Hep-PK/PBAN-R but not the Spl-PK/PBAN-R - a response opposite to that previously demonstrated with diapause hormone (DH). MT was somewhat more active on Spl-PK/PBAN-R than on Hep-PK/PBAN-R. LPK elicited similar positive responses in both receptors (like that with PBAN). A differential response toward both receptors was also noticed with the PBAN-derived backbone cyclic (BBC) conformationally constrained peptide BBC-5. The peptides BBC-7 and BBC-8 activated both receptors. The results correlate between two PK/PBAN mediated function (cuticular melanization and sex pheromone biosynthesis) and the peptides that activate them and thus advance our understanding of the mode of action of the PK/PBAN family, and might help in exploring novel high-affinity receptor-specific antagonists that could serve as a basis for development of new families of insect-control agents. © 2014 Elsevier Inc. All rights reserved.
Scientific Publication
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