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British Journal of Nutrition
Folman, Y., Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, MA, 02139, United States
Russell, R.M., USDA-Human Nutrition Research Center on Aging, Tufts University, MA, 02111, Boston, United States
Tang, G.W., USDA-Human Nutrition Research Center on Aging, Tufts University, MA, 02111, Boston, United States
Wolf, G., Department of Nutritional Sciences, University of California at Berkeley, Berkeley, CA 94720, United States
The aim of the present work was to determine the effect of giving increasing doses of supplements of β-carotene on serum retinoic acid levels in rabbits. Four groups of 7-week-old female rabbits were fed for 9 weeks on a pelleted diet containing 1.72 mg vitamin A as retinyl acetate/kg and including control gelatin beadlets devoid of β-carotene or 1, 2 or 4 mg β-carotene/kg body-weight per d. Serum was collected at 3, 6 and 9 weeks after the beginning of the experiment and the concentration of all-trans retinoic acid was determined by a gradient reverse-phase high-performance liquid chromatography system following a double-phase extraction. The average concentration of retinoic acid in serum of the combined control and 1 mg β-carotene/kg groups was 3.80, 3.06 and 2.40 nM at 3, 6 and 9 weeks respectively. The concentrations of retinoic acid in serum of the combined 2 and 4 mg V-carotene/kg groups were 4.80 nM (P < 0.05), 3.76 nM (not significant) and 4.90 nM (P < 0.005) at 3, 6 and 9 weeks respectively. A SAS (SAS Institute Inc., 1985) general linear model repeated-measures analysis of variance revealed that the effects of treatment (P < 0.01), time (P < 0.05) and treatment Χ time interaction (P < 0.05) were statistically significant. It is concluded that giving β-carotene is associated with higher concentrations of all-trans retinoic acid in the serum of rabbits than in those receiving no β-carotene. © 1989, The Nutrition Society. All rights reserved.
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Rabbits fed on β-carotene have higher serum levels of all-trans retinoic acid than those receiving no β-carotene
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Folman, Y., Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, MA, 02139, United States
Russell, R.M., USDA-Human Nutrition Research Center on Aging, Tufts University, MA, 02111, Boston, United States
Tang, G.W., USDA-Human Nutrition Research Center on Aging, Tufts University, MA, 02111, Boston, United States
Wolf, G., Department of Nutritional Sciences, University of California at Berkeley, Berkeley, CA 94720, United States
Rabbits fed on β-carotene have higher serum levels of all-trans retinoic acid than those receiving no β-carotene
The aim of the present work was to determine the effect of giving increasing doses of supplements of β-carotene on serum retinoic acid levels in rabbits. Four groups of 7-week-old female rabbits were fed for 9 weeks on a pelleted diet containing 1.72 mg vitamin A as retinyl acetate/kg and including control gelatin beadlets devoid of β-carotene or 1, 2 or 4 mg β-carotene/kg body-weight per d. Serum was collected at 3, 6 and 9 weeks after the beginning of the experiment and the concentration of all-trans retinoic acid was determined by a gradient reverse-phase high-performance liquid chromatography system following a double-phase extraction. The average concentration of retinoic acid in serum of the combined control and 1 mg β-carotene/kg groups was 3.80, 3.06 and 2.40 nM at 3, 6 and 9 weeks respectively. The concentrations of retinoic acid in serum of the combined 2 and 4 mg V-carotene/kg groups were 4.80 nM (P < 0.05), 3.76 nM (not significant) and 4.90 nM (P < 0.005) at 3, 6 and 9 weeks respectively. A SAS (SAS Institute Inc., 1985) general linear model repeated-measures analysis of variance revealed that the effects of treatment (P < 0.01), time (P < 0.05) and treatment Χ time interaction (P < 0.05) were statistically significant. It is concluded that giving β-carotene is associated with higher concentrations of all-trans retinoic acid in the serum of rabbits than in those receiving no β-carotene. © 1989, The Nutrition Society. All rights reserved.
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