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Precocious gladiolus corm formation in liquid shake cultures
Year:
1991
Authors :
Cohen, Avner
;
.
Goldberg, Zvia
;
.
Kochba, Marnina
;
.
Steinitz, Benjamin
;
.
Volume :
26
Co-Authors:
Steinitz, B., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Cohen, A., Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Goldberg, Z., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Kochba, M., Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Facilitators :
From page:
63
To page:
70
(
Total pages:
8
)
Abstract:
Conditions were defined for precocious differentiation and improved growth of corms at the base of gladiolus shoots. Shoots were derived from explants cultured on agar solidified media, and corm regeneration was obtained in subsequent liquid shake cultures. Benzyladenine (BA), at 10-7 M, was found to have a stimulating effect mainly when provided to the shoots prior to manifestation of corm growth. Paclobutrazol and sucrose promoted corm formation when supplemented to the liquid media. Paclobutrazol, at 10 mg l-1, shifted assimilate allocation towards the growing corm. A differential promotion of corm development by sucrose was not observed, and the concentration of sucrose at which the sugar demand for maximal shoot and corm growth is satisfied (60 g l-1) was unaltered by the presence of paclobutrazol. The rate of corm growth on shoots cultured in a liquid medium supplemented with paclobutrazol and a saturating sucrose concentration, was a function of the length of the shoot's leaf blades, and was similar in light and in dark. © 1991 Kluwer Academic Publishers.
Note:
Related Files :
corm
cytokinin
Gladiolus
paclobutrazol
sucrose
tissue culture
Show More
Related Content
More details
DOI :
10.1007/BF00036107
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24514
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:08
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Scientific Publication
Precocious gladiolus corm formation in liquid shake cultures
26
Steinitz, B., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Cohen, A., Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Goldberg, Z., Department of Plant Genetics, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Kochba, M., Department of Ornamental Horticulture, Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet Dagan, 50 250, Israel
Precocious gladiolus corm formation in liquid shake cultures
Conditions were defined for precocious differentiation and improved growth of corms at the base of gladiolus shoots. Shoots were derived from explants cultured on agar solidified media, and corm regeneration was obtained in subsequent liquid shake cultures. Benzyladenine (BA), at 10-7 M, was found to have a stimulating effect mainly when provided to the shoots prior to manifestation of corm growth. Paclobutrazol and sucrose promoted corm formation when supplemented to the liquid media. Paclobutrazol, at 10 mg l-1, shifted assimilate allocation towards the growing corm. A differential promotion of corm development by sucrose was not observed, and the concentration of sucrose at which the sugar demand for maximal shoot and corm growth is satisfied (60 g l-1) was unaltered by the presence of paclobutrazol. The rate of corm growth on shoots cultured in a liquid medium supplemented with paclobutrazol and a saturating sucrose concentration, was a function of the length of the shoot's leaf blades, and was similar in light and in dark. © 1991 Kluwer Academic Publishers.
Scientific Publication
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