נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Year:
2006
Authors :
Gafni, Yedidya
;
.
Volume :
344
Co-Authors:
Tzfira, T., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Kunik, T., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Gafni, Y., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Citovsky, V., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Facilitators :
From page:
435
To page:
451
(
Total pages:
17
)
Abstract:
Agrobacterium most likely can transform virtually all known plant species, and experimental protocols for Agrobacterium-mediated genetic transformation of yet more plant species, ecotypes, and cultivars are published almost on a daily basis. Interestingly, the Agrobacterium host range is not limited to the plant kingdom, and it has been shown to transform many species of fungi and even prokaryotes. The ability of Agrobacterium to genetically transform HeLa cells further widens the range of potential hosts of Agrobacterium to include humans and perhaps other animal species. Furthermore, because mammalian cells significantly differ from plant cells, they provide a useful experimental system for identification and functional characterization of plant-specific factors involved in the transformation process. Here, we present basic procedures for transfection and Agrobacterium-mediated genetic transformation of mammalian cells. We also demonstrate the use of mammalian cells for studies of the cellular components of the genetic transformation pathway.
Note:
Related Files :
Animal
Animals
Cell Culture Techniques
Genetics
genetic transformation
HeLa cells
human
Polymerase Chain Reaction
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24524
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:08
You may also be interested in
Scientific Publication
Mammalian cells.
344
Tzfira, T., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Kunik, T., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Gafni, Y., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Citovsky, V., Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, MI, USA.
Mammalian cells.
Agrobacterium most likely can transform virtually all known plant species, and experimental protocols for Agrobacterium-mediated genetic transformation of yet more plant species, ecotypes, and cultivars are published almost on a daily basis. Interestingly, the Agrobacterium host range is not limited to the plant kingdom, and it has been shown to transform many species of fungi and even prokaryotes. The ability of Agrobacterium to genetically transform HeLa cells further widens the range of potential hosts of Agrobacterium to include humans and perhaps other animal species. Furthermore, because mammalian cells significantly differ from plant cells, they provide a useful experimental system for identification and functional characterization of plant-specific factors involved in the transformation process. Here, we present basic procedures for transfection and Agrobacterium-mediated genetic transformation of mammalian cells. We also demonstrate the use of mammalian cells for studies of the cellular components of the genetic transformation pathway.
Scientific Publication
You may also be interested in