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Annals of Applied Biology
SPIEGEL, S., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
MARTIN, R.R., Agriculture Canada Research Station, 6660 N. W. Marine Drive, Vancouver, British Columbia, V6T 1X2, Canada
The polymerase chain reaction (PCR) method was reliably used for detection of potato leafroll virus (PLRV) RNA in dormant tubers from field‐grown plants and in vitro‐propagated microtubers. A simple RNA extraction from tuber and microtuber tissue allowed reverse transcription and PCR amplification of viral sequences. It was also demonstrated that ELISA could be used reliably for detection of PLRV in crude sap extracts of dormant microtubers produced in vitro but not in tubers from field‐grown plants. Copyright © 1993, Wiley Blackwell. All rights reserved
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Improved detection of potato leafroll virus in dormant potato tubers and microtubers by the polymerase chain reaction and ELISA
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SPIEGEL, S., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan, 50250, Israel
MARTIN, R.R., Agriculture Canada Research Station, 6660 N. W. Marine Drive, Vancouver, British Columbia, V6T 1X2, Canada
Improved detection of potato leafroll virus in dormant potato tubers and microtubers by the polymerase chain reaction and ELISA
The polymerase chain reaction (PCR) method was reliably used for detection of potato leafroll virus (PLRV) RNA in dormant tubers from field‐grown plants and in vitro‐propagated microtubers. A simple RNA extraction from tuber and microtuber tissue allowed reverse transcription and PCR amplification of viral sequences. It was also demonstrated that ELISA could be used reliably for detection of PLRV in crude sap extracts of dormant microtubers produced in vitro but not in tubers from field‐grown plants. Copyright © 1993, Wiley Blackwell. All rights reserved
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