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Cisgenic melons over expressing glyoxylate-aminotransferase are resistant to downy mildew
Year:
2009
Authors :
Kenigsbuch, David
;
.
Volume :
125
Co-Authors:
Benjamin, I., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Kenigsbuch, D., Department of Postharvest Science, Volcani Centre, Beit-Dagan 50250, Israel
Galperin, M., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Abrameto, J.A., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Cohen, Y., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Facilitators :
From page:
355
To page:
365
(
Total pages:
11
)
Abstract:
Downy mildew caused by the oomycete Pseudoperonospora cubensis, is a devastating foliar disease of cucurbits. The wild melon PI 124111F (PI) is highly resistant to this disease while BU21/3 and Hemed are susceptible. In a previous study we showed that resistance in PI is metabolic, resulting from enhanced activity of glyoxylate aminotransferase encoded by the genes At1 and At2. When either gene from PI was transformed into a susceptible plant it became resistant. Here we show that the nearly silent At1 and At2 in the susceptible Hemed confer resistance to downy mildew when overexpressed (by CaMV S35 promoter) in the susceptible BU21/3. The cisgenic plants, overexpressing either At1 or At2, exhibited enhanced activity of glyoxylate aminotransferase and resistance against P. cubensis. Northern and western gel blot analyses suggested that the low expression of At1 and At2 in the susceptible melons is modulated by transcriptional inhibition. © KNPV 2009.
Note:
Related Files :
Citrullus lanatus
climbing plant
Cucurbits
disease resistance
gene expression
genetic analysis
respiration
Show More
Related Content
More details
DOI :
10.1007/s10658-009-9485-4
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24539
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:08
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Scientific Publication
Cisgenic melons over expressing glyoxylate-aminotransferase are resistant to downy mildew
125
Benjamin, I., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Kenigsbuch, D., Department of Postharvest Science, Volcani Centre, Beit-Dagan 50250, Israel
Galperin, M., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Abrameto, J.A., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Cohen, Y., The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Cisgenic melons over expressing glyoxylate-aminotransferase are resistant to downy mildew
Downy mildew caused by the oomycete Pseudoperonospora cubensis, is a devastating foliar disease of cucurbits. The wild melon PI 124111F (PI) is highly resistant to this disease while BU21/3 and Hemed are susceptible. In a previous study we showed that resistance in PI is metabolic, resulting from enhanced activity of glyoxylate aminotransferase encoded by the genes At1 and At2. When either gene from PI was transformed into a susceptible plant it became resistant. Here we show that the nearly silent At1 and At2 in the susceptible Hemed confer resistance to downy mildew when overexpressed (by CaMV S35 promoter) in the susceptible BU21/3. The cisgenic plants, overexpressing either At1 or At2, exhibited enhanced activity of glyoxylate aminotransferase and resistance against P. cubensis. Northern and western gel blot analyses suggested that the low expression of At1 and At2 in the susceptible melons is modulated by transcriptional inhibition. © KNPV 2009.
Scientific Publication
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