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Nissan, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Yeheskel, A., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Pasmanik-Chor, M., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila. © 2012 The American Phytopathological Society.
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The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT
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Nissan, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Manulis-Sasson, S., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Chalupowicz, L., Department of Plant Pathology and Weed Research, ARO, Volcani Center, Bet Dagan 50250, Israel
Teper, D., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Yeheskel, A., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Pasmanik-Chor, M., Bioinformatics Unit, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Sessa, G., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
Barash, I., Department of Molecular Biology and Ecology of Plants, Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv 69978, Israel
The type III effector HsvG of the gall-forming Pantoea agglomerans mediates expression of the host gene HSVGT
The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila paniculata. HSVGT is a predicted acidic protein of the DnaJ family with 244 amino acids. It harbors characteristic conserved motifs of a eukaryotic transcription factor, including a bipartite nuclear localization signal, zinc finger, and leucine zipper DNA-binding motifs. Quantitative real-time polymerase chain reaction analysis demonstrated that HSVGT transcription is specifically induced in planta within 2 h after inoculation with the wild-type P. agglomerans pv. gypsophilae compared with the hsvG mutant. Induction of HSVGT reached a peak of sixfold at 4 h after inoculation and progressively declined thereafter. Gel-shift assay demonstrated that HsvG binds to the HSVGT promoter, indicating that HSVGT is a direct target of HsvG. Our results support the hypothesis that HsvG functions as a transcription factor in gypsophila. © 2012 The American Phytopathological Society.
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