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Akavia, N., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany, Deutschen Akademischen Austauschdienstes, Department o f Floriculture and Ornamental Plants, Volcani Center, Bet Dagan, Israel
Strack, D., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany
The described method allows rapid screening and accurate quantification of anthocyanidins with the aid of HPLC. Using a linear gradient in 40 min from 20 to 40% of solvent B (1.5% phosphoric acid – 20% acetic acid – 25% acetonitrile) in solvent A (1.5% phosphoric acid) + B on LiChrosorb RP-8 the common six anthocyanidins delphinidin, cyanidin, petunidin, pelargonidin, peonidin, and malvidin were resolved within 40 min. It is demonstrated that acetonitrile is superior to other solvents in resolving methoxylated phenolic compounds. The application of the described method is shown with Gladiolus petals in studies of flower pigment genetics. © 1980, Walter de Gruyter. All rights reserved.
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High Performance Liquid Chromatography of Anthocyanidins as a New Approach to Study Flower Pigment Genetics
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Akavia, N., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany, Deutschen Akademischen Austauschdienstes, Department o f Floriculture and Ornamental Plants, Volcani Center, Bet Dagan, Israel
Strack, D., Botanisches Institut der Universität zu Köln, Gyrhofstr. 15, D-5000 Köln 41, Germany
High Performance Liquid Chromatography of Anthocyanidins as a New Approach to Study Flower Pigment Genetics
The described method allows rapid screening and accurate quantification of anthocyanidins with the aid of HPLC. Using a linear gradient in 40 min from 20 to 40% of solvent B (1.5% phosphoric acid – 20% acetic acid – 25% acetonitrile) in solvent A (1.5% phosphoric acid) + B on LiChrosorb RP-8 the common six anthocyanidins delphinidin, cyanidin, petunidin, pelargonidin, peonidin, and malvidin were resolved within 40 min. It is demonstrated that acetonitrile is superior to other solvents in resolving methoxylated phenolic compounds. The application of the described method is shown with Gladiolus petals in studies of flower pigment genetics. © 1980, Walter de Gruyter. All rights reserved.
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