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Characterization of Alternaria alternata causing black spot disease of pomegranate in Israel using a molecular marker
Year:
2012
Source of publication :
Plant Disease
Authors :
Ezra, David
;
.
Gat, Tami
;
.
Liarzi, Orna
;
.
Skovorodnikova, Yulia
;
.
Volume :
96
Co-Authors:
Gat, T., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Liarzi, O., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Skovorodnikova, Y., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Ezra, D., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
1513
To page:
1518
(
Total pages:
6
)
Abstract:
Black spot disease of pomegranate is a relatively new disease in Israel that is caused by Alternaria alternata. The symptoms include black spots on leaves and fruit. Only the outer part of the fruit is damaged; the edible tissue remains unaffected. In this study, we obtained 50 isolates of A. alternata from infected pomegranate plants that were classified based on pathogenicity tests using detached leaves. Using an arbitrarily primed polymerase chain reaction, we identified one primer (primer CAG) that reacted only with DNA of isolates that induced the most severe disease symptoms. Based on the sequence of the amplified fragment, we generated a specific primer (primer C) that recognizes these highly virulent isolates. Therefore, we suggest that primer C can be utilized as a molecular marker for the detection of A. alternata isolates that cause black spot disease of pomegranate. © 2012 The American Phytopathological Society.
Note:
Related Files :
Alternaria alternata
Lythraceae
Show More
Related Content
More details
DOI :
10.1094/PDIS-12-11-1041-RE
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24676
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
Scientific Publication
Characterization of Alternaria alternata causing black spot disease of pomegranate in Israel using a molecular marker
96
Gat, T., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Liarzi, O., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Skovorodnikova, Y., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Ezra, D., Department of Plant Pathology and Weed Research, ARO The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Characterization of Alternaria alternata causing black spot disease of pomegranate in Israel using a molecular marker
Black spot disease of pomegranate is a relatively new disease in Israel that is caused by Alternaria alternata. The symptoms include black spots on leaves and fruit. Only the outer part of the fruit is damaged; the edible tissue remains unaffected. In this study, we obtained 50 isolates of A. alternata from infected pomegranate plants that were classified based on pathogenicity tests using detached leaves. Using an arbitrarily primed polymerase chain reaction, we identified one primer (primer CAG) that reacted only with DNA of isolates that induced the most severe disease symptoms. Based on the sequence of the amplified fragment, we generated a specific primer (primer C) that recognizes these highly virulent isolates. Therefore, we suggest that primer C can be utilized as a molecular marker for the detection of A. alternata isolates that cause black spot disease of pomegranate. © 2012 The American Phytopathological Society.
Scientific Publication
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