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Characterisation of the chromosome fusions in Oreochromis karongae
Year:
2010
Source of publication :
Chromosome Research
Authors :
Shirak, Andrey
;
.
Volume :
18
Co-Authors:
Mota-Velasco, J.C., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom, Landcatch Natural Selection, E.Centre, Cooperage Way Business Village, Alloa, FK10 3LP Clackmannanshire, United Kingdom
Ferreira, I.A., Departamento de Morfologia, Instituto de Biociências, UNESP-Universidade Estadual Paulista, 18618-970 Botucatu, SP, Brazil
Cioffi, M.B., Departamento de Genética e Evolução, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, 13565-905 São Carlos, SP, Brazil
Ocalewicz, K., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom, Department of Ichthyology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
Campos-Ramos, R., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom, Centro de Investigaciones Biológicas Del Noroeste S.C. (CIBNOR), La Paz, Mexico
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Lee, B.-Y., Department of Biology, University of Maryland, 2132 Biosciences Research Building, College Park, MD 20742, United States, Laboratory of Bioinformatics and Population Genetics, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea
Martins, C., Departamento de Morfologia, Instituto de Biociências, UNESP-Universidade Estadual Paulista, 18618-970 Botucatu, SP, Brazil
Penman, D.J., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom
Facilitators :
From page:
575
To page:
586
(
Total pages:
12
)
Abstract:
Oreochromis karongae, one of the "chambo" tilapia species from Lake Malawi, has a karyotype of 2n=38, making it one of the few species investigated to differ from the typical tilapia karyotype (2n=44). The O. karongae karyotype consists of one large subtelocentric pair of chromosomes, four mediumsized pairs (three subtelocentric and one submetacentric) and 14 small pairs. The five largest pairs could be distinguished from each other on the basis of size, morphology and a series of fluorescence in situ hybridisation (FISH) probes. The largest pair is easily distinguished on the basis of size and a chromosome 1 (linkage group 3) bacterial artificial chromosome (BAC) FISH probe from Oreochromis niloticus. BAC clones from O. niloticus chromosome 2 (linkage group 7) hybridised to one of the mediumsized subtelocentric chromosome pairs (no. 5) of O. karongae, distinguishing the ancestral mediumsized pair from the three other medium-sized chromosome pairs (nos. 2, 3 and 4) that appear to have resulted from fusions. SATA repetitive DNA hybridised to the centromeres of all 19 chromosome pairs and also revealed the locations of the relic centromeres in the three fused pairs. Telomeric (TTAGGG)n repeats were identified in the telomeres of all chromosomes, and an interstitial telomeric site (ITS) was identified in three chromosomal pairs (no. 2, 3 and 4). Additionally, two ITS sites were identified in the largest chromosome pair (pair 1), confirming the origin of this chromosome from three ancestral chromosomes. SATA and ITS sites allowed the orientation of the fusions in pairs 2, 3 and 4, which all appear to have been in different orientations (q-q, p-q and p-p, respectively). One of these fusions (O. karongae chromosome pair no. 2) involves a small chromosome (equivalent to linkage group 1), which in O. niloticus carries the main sex-determining gene. 4′,6-Diamidino-2-phenyloindole staining of the synaptonemal complex in male O. karongae revealed the presumptive positions of the kinetochores, which correspond well to the centromeric positions observed in the mitotic karyotype. © 2010 Springer Science+Business Media B.V.
Note:
Related Files :
Animals
Female
fish
Genetics
Male
Oreochromis niloticus
Tilapia
Show More
Related Content
More details
DOI :
10.1007/s10577-010-9141-z
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24691
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
Scientific Publication
Characterisation of the chromosome fusions in Oreochromis karongae
18
Mota-Velasco, J.C., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom, Landcatch Natural Selection, E.Centre, Cooperage Way Business Village, Alloa, FK10 3LP Clackmannanshire, United Kingdom
Ferreira, I.A., Departamento de Morfologia, Instituto de Biociências, UNESP-Universidade Estadual Paulista, 18618-970 Botucatu, SP, Brazil
Cioffi, M.B., Departamento de Genética e Evolução, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, 13565-905 São Carlos, SP, Brazil
Ocalewicz, K., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom, Department of Ichthyology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
Campos-Ramos, R., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom, Centro de Investigaciones Biológicas Del Noroeste S.C. (CIBNOR), La Paz, Mexico
Shirak, A., Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Lee, B.-Y., Department of Biology, University of Maryland, 2132 Biosciences Research Building, College Park, MD 20742, United States, Laboratory of Bioinformatics and Population Genetics, Department of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea
Martins, C., Departamento de Morfologia, Instituto de Biociências, UNESP-Universidade Estadual Paulista, 18618-970 Botucatu, SP, Brazil
Penman, D.J., Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, United Kingdom
Characterisation of the chromosome fusions in Oreochromis karongae
Oreochromis karongae, one of the "chambo" tilapia species from Lake Malawi, has a karyotype of 2n=38, making it one of the few species investigated to differ from the typical tilapia karyotype (2n=44). The O. karongae karyotype consists of one large subtelocentric pair of chromosomes, four mediumsized pairs (three subtelocentric and one submetacentric) and 14 small pairs. The five largest pairs could be distinguished from each other on the basis of size, morphology and a series of fluorescence in situ hybridisation (FISH) probes. The largest pair is easily distinguished on the basis of size and a chromosome 1 (linkage group 3) bacterial artificial chromosome (BAC) FISH probe from Oreochromis niloticus. BAC clones from O. niloticus chromosome 2 (linkage group 7) hybridised to one of the mediumsized subtelocentric chromosome pairs (no. 5) of O. karongae, distinguishing the ancestral mediumsized pair from the three other medium-sized chromosome pairs (nos. 2, 3 and 4) that appear to have resulted from fusions. SATA repetitive DNA hybridised to the centromeres of all 19 chromosome pairs and also revealed the locations of the relic centromeres in the three fused pairs. Telomeric (TTAGGG)n repeats were identified in the telomeres of all chromosomes, and an interstitial telomeric site (ITS) was identified in three chromosomal pairs (no. 2, 3 and 4). Additionally, two ITS sites were identified in the largest chromosome pair (pair 1), confirming the origin of this chromosome from three ancestral chromosomes. SATA and ITS sites allowed the orientation of the fusions in pairs 2, 3 and 4, which all appear to have been in different orientations (q-q, p-q and p-p, respectively). One of these fusions (O. karongae chromosome pair no. 2) involves a small chromosome (equivalent to linkage group 1), which in O. niloticus carries the main sex-determining gene. 4′,6-Diamidino-2-phenyloindole staining of the synaptonemal complex in male O. karongae revealed the presumptive positions of the kinetochores, which correspond well to the centromeric positions observed in the mitotic karyotype. © 2010 Springer Science+Business Media B.V.
Scientific Publication
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