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Effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro
Year:
2003
Source of publication :
Cryo-Letters
Authors :
Gafny, Ron
;
.
Mawassi, Munir
;
.
Volume :
24
Co-Authors:
Wang, Q., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Li, P., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Batuman, Ö., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gafny, R., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Mawassi, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
293
To page:
302
(
Total pages:
10
)
Abstract:
The effect of N6-benzyladenine (BA) on the recovery of cryopreserved shoot tips of the LN33 hybrid (Vitis L.) and 'Troyer' citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis {L.} Osbeck.] cultured in vitro was examined. For the LN33 hybrid, the presence of BA in the recovery medium was essential for survival of control and cryopreserved shoot tips, although the BA concentration did not influence the survival percentage. BA at 5, 2, and 5 μM or higher induced callus formation in control, and shoot tips cryopreserved by vitrification, and by encapsulation-dehydration, respectively. While a BA concentration of 4 μM was found optimal for recovery of control shoot tips, 1 and 2-4 μM produced the best recovery of shoot tips cryopreserved by vitrification and encapsulation-dehydration, respectively. A similar pattern of effect of BA on recovery was found for 'Troyer' citrange. Low survival of control and cryopreserved shoot tips was observed with a BA-free recovery medium. The addition of BA to the recovery medium significantly increased survival. The BA concentration that induced callus formation in shoot tips cryopreserved by encapsulation-vitrification was higher than that which induced it in those cryopreserved by encapsulation-dehydration. Recovery of control shoot tips was best with an addition of 6-10 μM BA to the medium. Optimal recovery of shoot tips cryopreserved by encapsulation- vitrification and encapsulation-dehydration was achieved with 3-4 and 2 μM BA, respectively. Results from the present study suggest that an optimal BA concentration for recovery of control shoot tips may be different from that for cryopreserved shoot tips; furthermore, the optimal BA concentration for recovery of cryopreserved shoot tips may also differ among different cryogenic procedures.
Note:
Related Files :
adenine
Citrus sinensis
Cryoprotective Agents
kinetin
Plant Shoots
Poncirus trifoliata
Shoot tip
Vitis
Vitrification
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24739
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:09
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Scientific Publication
Effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro
24
Wang, Q., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Li, P., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Batuman, Ö., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Gafny, R., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Mawassi, M., Department of Virology, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Effect of benzyladenine on recovery of cryopreserved shoot tips of grapevine and citrus cultured in vitro
The effect of N6-benzyladenine (BA) on the recovery of cryopreserved shoot tips of the LN33 hybrid (Vitis L.) and 'Troyer' citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis {L.} Osbeck.] cultured in vitro was examined. For the LN33 hybrid, the presence of BA in the recovery medium was essential for survival of control and cryopreserved shoot tips, although the BA concentration did not influence the survival percentage. BA at 5, 2, and 5 μM or higher induced callus formation in control, and shoot tips cryopreserved by vitrification, and by encapsulation-dehydration, respectively. While a BA concentration of 4 μM was found optimal for recovery of control shoot tips, 1 and 2-4 μM produced the best recovery of shoot tips cryopreserved by vitrification and encapsulation-dehydration, respectively. A similar pattern of effect of BA on recovery was found for 'Troyer' citrange. Low survival of control and cryopreserved shoot tips was observed with a BA-free recovery medium. The addition of BA to the recovery medium significantly increased survival. The BA concentration that induced callus formation in shoot tips cryopreserved by encapsulation-vitrification was higher than that which induced it in those cryopreserved by encapsulation-dehydration. Recovery of control shoot tips was best with an addition of 6-10 μM BA to the medium. Optimal recovery of shoot tips cryopreserved by encapsulation- vitrification and encapsulation-dehydration was achieved with 3-4 and 2 μM BA, respectively. Results from the present study suggest that an optimal BA concentration for recovery of control shoot tips may be different from that for cryopreserved shoot tips; furthermore, the optimal BA concentration for recovery of cryopreserved shoot tips may also differ among different cryogenic procedures.
Scientific Publication
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